Short on time?

Get essay writing help

Use Of Chromatography To Study Pigments From Leaf Extracts

  • Words: 478
  • |
  • Page: 1
  • This essay sample was donated by a student to help the academic community. Papers provided by EduBirdie writers usually outdo students' samples.

Introduction

According to Giddings and Keller (2016), chromatography is a technique used to separate solutes from a mixture. It is widely used for separation and identification of biological compounds, or to analyse mixtures. An example of its use is separation of pigments from dyes. Identification of compounds is done by using retention factor values, simply known as Rf values. The objective of this experiment is to separate the pigments of chlorophyll, from leaves from four different species of plants, namely Morus alba, Cissus species, Bougainvillea species and Hibiscus species. As stated by Raven (2009), chlorophyll is the pigment found in plants, some bacteria, and some algae. It absorbs light necessary for photosynthesis and it is a large molecule that is composed of mainly carbon and hydrogen. As Gida (2011) had mentioned, the name chlorophyll is derived from the Greek words chloros (green) and phyllon (leaf). Raven (2009) continued to mention that there are different types of chlorophyll and that the different types of chlorophyll absorb different wavelengths of light. Elliott (2019) mentions five different types of chlorophyll: A, B, C, D, and E.

Save your time!
We can take care of your essay
  • Proper editing and formatting
  • Free revision, title page, and bibliography
  • Flexible prices and money-back guarantee
Place Order

Materials and method

1.5g of leaves from each type of plant (Morus alba, Cissus species, Bougainvillea species and Hibiscus species) was measured. 0.5g of anhydrous MgSO4 and 1g of acid washed sand, as well as 1.5g of leaves from one type of plant was added into a pestle and mortar. They were grinded into a fine mixture before having 10ml of acetone added to the mixture, mixing for under 10s. the supernatant was then quickly added into a centrifuge tube and vortex for 30s. Afterwards, it was placed in a centrifuge for 10 minutes at 2300rpm, then the supernatant was collected into an eppendorf. The above steps were repeated with the other three leaf samples. 20ml of the solvent (containing cyclohexane, petroleum ether, ethyl acetate, acetone and methanol in the ratio; 16:60:10:10:4) was added into a chromatography tank, then closed immediately. Using a pencil, a line 1.5cm from the bottom of the chromatography plate was drawn. Marks were made at 2cm intervals along the line, starting from the left-hand side. Using a capillary tube, the extracts were applied at the marks made along the line, making sure the spot does not exceed a diameter of 5mm or pass a maximum of five drops of each extract. The chromatography plate was then carefully placed into the development tank and covered. Once the solvent front covers 80% of the chromatography plate, it was removed and a line was carefully drawn along the wetting front while still wet.

References

  1. J. Calvin Giddings, and Roy. A Keller, (2016) Chromatography, https://www.britannica.com/science/chromatography
  2. Peter H. Raven, and Marshall R. Crosby, Chlorophyll Microsoft® Encarta® Online Encyclopedia (2009), https://www.refseek.com/data/cache/en/1/Chlorophyll.html
  3. Walsh, Elliot, (2019, October 19). Types of Chlorophyll Present in Algae. sciencing.com. Retrieved from https://sciencing.com/types-chlorophyll-present-algae-8433014.html
  4. A. Levent İnanç, (2011), Chlorophyll: Structural Properties, Health Benefits and Its Occurrence in Virgin Olive Oils, http://www.academicfoodjournal.com/archive/2011/issue2/26-32.pdf

Make sure you submit a unique essay

Our writers will provide you with an essay sample written from scratch: any topic, any deadline, any instructions.

Cite this Page

Use Of Chromatography To Study Pigments From Leaf Extracts. (2022, February 17). Edubirdie. Retrieved December 1, 2022, from https://edubirdie.com/examples/use-of-chromatography-to-study-pigments-from-leaf-extracts/
“Use Of Chromatography To Study Pigments From Leaf Extracts.” Edubirdie, 17 Feb. 2022, edubirdie.com/examples/use-of-chromatography-to-study-pigments-from-leaf-extracts/
Use Of Chromatography To Study Pigments From Leaf Extracts. [online]. Available at: <https://edubirdie.com/examples/use-of-chromatography-to-study-pigments-from-leaf-extracts/> [Accessed 1 Dec. 2022].
Use Of Chromatography To Study Pigments From Leaf Extracts [Internet]. Edubirdie. 2022 Feb 17 [cited 2022 Dec 1]. Available from: https://edubirdie.com/examples/use-of-chromatography-to-study-pigments-from-leaf-extracts/
copy
Join 100k satisfied students
  • Get original paper written according to your instructions
  • Save time for what matters most
hire writer

Fair Use Policy

EduBirdie considers academic integrity to be the essential part of the learning process and does not support any violation of the academic standards. Should you have any questions regarding our Fair Use Policy or become aware of any violations, please do not hesitate to contact us via support@edubirdie.com.

Check it out!
close
search Stuck on your essay?

We are here 24/7 to write your paper in as fast as 3 hours.