University:
Boston University
Course:
CAS CH 101 | General Chemistry 1
Academic year:

2020
Views:

181

Pages:

2
Author:

Alia Aguirre

Phosphor Imaging with the Sapphire Biomolecular Imager Introduction Results and Conclusions While fluorescent and chemiluminescent imaging techniques and their detectors are becoming more sensitive and selective, photostimulated luminescence from phosphor storage plates remains a key application for many researchers, especially for applications such as electrophoretic mobility shift assays (EMSA), enzyme assays, and in vivo imaging. The Sapphire Biomolecular Imager captures high quality images with a large dynamic range from standard storage phosphor screens (Figure 1). Following sample quantification using AzureSpot analysis software, the LOD for American Radiolabeled Chemicals 14C standard after a 3-hour exposure was determined to be 0.036 μCi/g. Furthermore, a highly linear dynamic range from 1000 to 0.036 μCi/g was observed (r2 = 0.99 [Figure 2]). Traditional phosphor imagers are often single function and require a large footprint. The Azure Biosystems Sapphire Biomolecular Imager offers the ability to scan storage phosphor screens for filmless autoradiography, with exceptional dynamic range and image quality thanks to the use of laser excitation and photon multiplier tube (PMT) detection. The compact Sapphire Biomolecular Imager also incorporates exceptional visible fluorescent and near infra-red scanning along with true chemiluminescent and white light imaging making it a true lab workhorse. To conclude, the Sapphire Biomolecular Imager is capable of imaging storage phosphor screens producing high quality images for later quantification; while also offering the ability to rapidly image fluorescent, chemiluminescent and NIR samples all within a single compact footprint. 0.036 4.5 µCi/g Materials and Methods An American Radiolabeled Chemicals 14C standard with slices from 1000 to 0.004 μCi/g was exposed to a BAS-MS storage phosphor screen (0.9 DPM/mm2/hr sensitivity) for 3 hours. Storage Phosphor Plate Imaging Following exposure, the storage phosphor screen was imaged using the Phosphor Imaging module of the Sapphire Biomolecular Imager with standard excitation and detection settings. Samples were quantified and limits of detection (LOD) and dynamic range (DR) calculated. Figure 1. American Radiolabeled Chemicals Carbon-14 standard exposed to Storage Phosphor Screen for three hours then imaged on the Sapphire Biomolecular Imager. 5 Log Integrated Intensity Sample Preparation 4 3 2 1 0 1 2 3 4 5 6 Log nCi/g Figure 2. Sample: 14C autoradiographic standard; Imaging: Phosphor; LOD: 0.036 µCi/g; DR: 5.4 orders of magnitude; Linearity: R2=0.99 Step Product Part number Sample preparation American Radiolabeled Chemicals – 14C standard ARC0146F BAS-IP-MS – Storage phosphor screen IS1011 Cassette for Phosphor Imaging IS1012 Azure Biosystems – Sapphire Biomolecular Imager with Phosphor Imaging Upgrade IS1002, IS1004 Storage phosphor plate Phosphor plate imaging Table 1. Materials and product numbers.