University:
Brigham Young University
Course:
CHEM 553 | Advanced Organic Chemistry
Academic year:

2022
Views:

497

Pages:

17
Author:

CometCrafter

Unit-1 principle of microscopy!. Der microscope can see the and is an instrument, by which we mecroorganisms, which are very small can't be seen through to co naked eye. microscopy microscopy is the to view objects & areas Science seen through naked eye. of wan wing microscopes of objects that can't be Cameroscope awas Zaccharian Come which Janseen make the Compound micro scope earliest can used two ens init). A.V. Leewenhoek 104 wef microscope. There are 2 tuper to mecroscopy te Light microscope (Optical microscope) ② Electron microscope. 2 Light light microscope use - optical lens & light. ✓ Electron microscope use. electromagnetic lens fa sriot beam of electrons Types of microscopy? i-e There are 2 mafor typer ds microscopy @optical/Light microscope (iⅱ. Electron microscope ①Light microscope! →gtis a system in which, magnification is obtained by wing an optical lene help of a light wave. Mereth the →Again itis devided into 2. tupes- @simple microsiope compand microscope. Simple micronupe simple microscope is consist ofy bi-conven lens Snort focal length. with a Compond microscope compound microscope microscope is a high magnification which use level of magnification 2 lentes to enhance the →1st lens is called objective lens of the and lens is Loud as the eye piece. Electon mecroscope! → gt is an system of Arrangement of electromagnetic lens with a short beam of electrom used to obtain magnification. at is again 2 tuper @Transmission election microscope (TEM) Scanning electron microscope. Cryotination: SM Cryotination is a proces Of cell architecture. of preservation 97 consist of breezing the sample Quickly without crystal tormation. voy of preserve the internal all thuid phase to solid. Exezing truckere by The method involve ultra-rapid cooline of. Sample i-e below. temput liquid nitrogen (-196°c) orNegative Staining SN staining is a procen used to enhance the bidorical spelimen at microscopic level. Negative staining is done to study the spelimen such as vines, bacteria ete. with •om this technique, the baground is stained heavy metal salts (eg:- Ammonicem, may bedate etc) These heavy metals do not penetrate into the Specimen & produced dark baground bagend fuhre brighter image formed. This staining procen is used, so that the electron beam can pay through the siperimeneasily, than surrounding spale. Shadow Casting SN This technique is ued to improve the contrast specimen. m this techniane, metal conding dary m the specimen. Suitable metals are. The arla which areal with no stain looke gold, chromicum etc. stain is very dark f bn gnt-