University:
California State University, Northridge
Course:
CHEM 464 | Principles of Biochemistry
Academic year:

2024
Views:

500

Pages:

1
Author:

Uszewskadndr

Enzyme worksheet question and answers 1. Using only the data in the above graph, outline the effect of increasing fructose 6-phosphate concentration on the activity of phosphofructokinase, at a low ATP concentration. (a) (i) increasing fructose 6-phosphate concentration (initially) causes an increase in activity; activity levels out / remains constant as (substrate) concentration continues to rise; if ATP already available; 2. Explain how increases in fructose 6-phosphate concentration affect the activity of the enzyme. (ii) more collisions with active site as concentration rises; at high substrate levels all active sites are occupied so no further increase in rate / enzyme working at maximum rate; 3. Outline the effect of increasing the ATP concentration on the activity of phosphofructokinase. (b) (i) decreases activity; at all fructose 6-phosphate concentrations; most effect at intermediate fructose 6-phosphate concentrations / little difference at high fructose 6-phosphate concentrations; ATP acts as an inhibitor; 2 max 4. Suggest an advantage to liv- (ii) end-product inhibition; ing organisms of the effect of respiration rate decreased ATP on phosphofructokinase. 1/1