Antibiogram Lecture Note

University:
Franklin University
Course:
EXS-351 | Kinesiology/Biomechanics
Academic year:

2020
Views:

151

Pages:

21
Author:

AuroraArcher

Antibiogram 1 Introduction  Goal of antimicrobial susceptibility testing  Predict in vivo success or failure of antibiotic therapy  Main purposes:  As a guide for treatment  As an epidemiological tool  Emergence of resistant strains of major pathogens (e. g. Shigella, Salmonella typhi)  Continued surveillance of susceptibility pattern of prevalent strains (e. g. Staphylococci, Gram negative Bacilli) 2 What Does Laboratory Need to Know about Antimicrobial Susceptibility Testing ?  Which organisms to test?  What methods to use?  What antibiotics to test?  How to report results? 3 Routine Susceptibility Tests  Disk diffusion (Kirby Bauer)  MIC  Broth microdilution  Broth macrodilution  E-test Basic sets of drugs for routine susceptibility tests 5 Susceptibility Testing Methods 6 Disk Agar Diffusion Test (DAD) (Kirby-Bauer test)  Procedure (Modified Kirby-Bauer method)  NCCLS  National Committee for Clinical Laboratory Standards  CLSI  Clinical and laboratory standards institute 7 Disk Diffusion Test (Kirby-Bauer test ) 9 Disk Agar Diffusion Test (DAD) 10 Disk Diffusion Test 11 Disk Diffusion Test 12 Incubate Overnight 13 Reading Results 14 Reporting Results 15 Dilution Method  Minimum Inhibition Concentration (MIC)  Lowest concentration of antimicrobial agent that inhibits bacterial growth/ multiplication  Minimum Bactericidal Concentration (MBC) or  Minimum Lethal Concentration (MLC) 16 MIC S. S. Khoramrooz 17 MIC and MBC 18 Micro-broth dilutions 19 E- test MIC on a strip 20 E- test 21 Clinical Conditions when MICs are Useful  Endocarditis  Meningitis  Septicemia  Osteomyelitis  Immunosuppressed patients (HIV, cancer, etc.)  Prosthetic devices  Patients not responding despite “S” Reports 22