Bioelectrochem: From Cells to Sensors

Bioelectrochem: From Cells to Sensors Detection of Neurochemicals Small organic makeudes that participate in neural activity and are chemical messengers. What thes overall is still poorly understood, most are redox active and be detected directly with electrochemistry Dopamine Perhaps the most widely studied neurotransmitter, essential for astablishment of reward based behaviours Depletion indicand as a marker in Parkinson's and Albaimer's, which relates to the increase in dopamine caused by amphetamines How Neurochemicale Transmit Information HD. 26+24 Serotonin Arunspores Receptor Tryplanten HO Phe syamenc Newrong Seroton Synthes Blerabdism Storage Detection of Dopamine Extra eldar fluid The release of dopamine from pale ogoura sia exceytosis, within a milisecond fimascule process, meaning the sensors must be font, sanafive, selictive and biocompubl Reuploade Clearing clas Avan (dan) Syrophe 687 Carbon Fibre Micro-slectrodas Cytoplasm CFME's ans electrodes in which carbon fibres serve as the alietro active arsa Adantages include Biologically compatible to col -Smalize thanum in diametere alowing implantation in HO Scan alectrode from a holding potential to a suitching potential and back at a high sman rate. Repeat assane every 100ms. As potential is inonused, dopamine is Fast-unan Cyelke Voltamery Coidised to dopamine-o-quinons. This is rearsed as potential daertains HO HO Neurochemicals aru Metaboksed by Radox Eneym Monoamin IMAO, aldehyde HO Catechol-matte francICOMT) Cahvehel-O-methyl-transfur(COMTI OH HO sor Moncamin coodae IMAOL aldehyde dehydrogenase Dopamine (DA Dopamine-o-quinone Radox Chemistry of Neurochemicala Dopamine, serotonin and their metabolites are alandantive. These types of MC's typostcandy proton coupled ston at the catechol or phenol sites, theu mune that we can detect them wing dichoshuning Many biomokoudes that undergo reduction/qidation do so simultaneously with proton mechaniem which int fully understood Important consequenc 26+24 HO HO Dydlic voltammograma ↓ Faust soun nat cause lange background charging cuttend dun to changing the double layer When dopamine is added fred inel, the differences an amal HO 26 HO Background-suttracted cyclic voltannoyram of dopamins ↓ The background current is stable and can be subtracted out to obtain a background aubimacted out to obtain a background-subtracted cycle voltammogram for dopamine The position of the peaks help identify the moknude being detected