CHEMIOSMOSIS &OXIDATIVEATP SYTHESIS AIthe protons that accumulate in the intermembrane spaceduring ETC create the electrochemical gradient thatstores free energythe free energy stored moves through an ATPase complex,using a proton-motive force (PMF)free energy is reduced and creates ATP from ADP + P;intermembraneHH*4'CHEMIOSMOSIS )a process for synthesizing ATPATPueusing the energy of anelectrochemical gradient andthe ATP synthase enzymeAby AATPmatrixalthough some of the energy dissipates as heat, much ofit is captured by condensing ADP and Pi into ATP at ATPasecontinual production of ATP requires H+ resevoirH+ resevoir requires continual movement of H+movement of ETC requires oxygen as final e- acceptor[ UNCOUPLING ETC & CHEMIOSMOSIS Iwhen uncoupled, energy is released as thermal energyusing specific uncoupling proteins that do not produce ATPuncoupling proteins is high in certain tissues such as fatsuncoupling causes free energy to help regulate body temperature