University:
Lake Sumter State College
Course:
MCB 2010C | Microbiology w/Lab
Academic year:

2022
Views:

438

Pages:

1
Author:

cravelingstnw

Question 1 Part III The hospital microbiology laboratory presumptively identifies organisms as GAS if they are 1) beta-hemolytic on sheep blood agar, 2) sensitive to bacitracin, 3) CAMPfactor* negative, 4) inhibited by 6.5% NaC1 broth, and 5) bile-esculin negative. These techniques were not changed during last two years. Microbial susceptibilities are performed by the method of Kirby-Bauer. Table 2 lists additional surveillance/laboratory data and denominator data. TABLE 2 Surveillance/Laboratory and Denominator Data # Surgical Surgical Wound Wound Infections OR Hospital Infection RATE Month Total Grp A Strep Procedures Discharges Total Grp A Strep 1979 January 5 0 600 2950 ______ ______ February 5 0 590 2900 ______ ______ March 2 0 580 2750 ______ ______ April 2 0 560 2520 ______ ______ May 6 0 600 2700 ______ ______ June 3 0 530 2400 ______ ______ July 3 0 520 2450 ______ ______ August 3 0 490 2530 ______ ______ September 4 0 560 2690 ______ ______ October 3 0 580 2730 ______ ______ November 2 0 590 2860 ______ ______ December NA NA 500 2640 ______ ______ 1980 January NA NA 590 2970 ______ ______ February 7 0 600 2890 ______ ______ March 10 0 590 2710 ______ ______ April 10 1 620 2630 ______ ______ May 17 5 610 2720 ______ ______ June 8 0 540 2660 ______ ______ July 13 3 560 2610 ______ ______ Aug. 1-15 3 1 270 1340 ______ ______ * A substance produced by Group B Streptococci which enhances the beta-lysin production of Staphylococcus aureus. QUESTION 7 a: Calculate the monthly rate (per 1000) of all surgical wound infections and of Group A Streptococcal surgical wound infections. Graph your findings. QUESTION 7b: Interpret these findings. Based on the information you have at this point, do you think an epidemic exists? QUESTION 8: Review your answers to Question 2. Which of the explanations can be eliminated at this point? Answer Part III The hospital microbiology laboratory presumptively identifies organisms as GAS if they are 1) beta-hemolytic on sheep blood agar, 2) sensitive to bacitracin, 3) CAMPfactor* negative, 4) inhibited by 6.5% NaC1 broth, and 5) bile-esculin negative. These techniques were not changed during last two years. Microbial susceptibilities are performed by the method of Kirby-Bauer. Table 2 lists additional surveillance/laboratory data and denominator data. TABLE 2 Surveillance/Laboratory and Denominator Data