University:
Lehman College
Course:
BIO 230 | Microbiology
Academic year:

2022
Views:

191

Pages:

19
Author:

Kasey Bruce

NC Pc SP 160 Sp 120 Western Blot Detect specific Proteins Within a mixture P68 P55 8041 P40 Σχ Hiv P34 P24 NC Pc P17 COM Plement Use Fixation YYY Serum Antibodies Antigen bind with Antibodies ९९९ Complement Binds with Ag-Ab Complex Add RBCS "indicator" Quantitate Ag-Ab Reactions. Serum without Antibodies Unbound Antigen Un bound Complement 8 Add RBCS "indicator" 8 No Hemolysis Hemolysis By Unbound Complement Lateral Flow fest immuno chromatographic assay" Test Sample Pows Via Capillary action. Ex Pregnancy test. Sample Solution containing target Analyte [AAA] Conjugate Pad str. Analyte: Antibodies Test Live Control Line AAA+ Analyte = #ve test Anti-AAA always +Ve 17 Flow Lytometry Cells Labelled with Fluorescent dye. Applications →Separate Cells. Measure DNA amount identify stage of growth Lyrle Detect genonormalities. Laser Forward and Side Scattered Light From all cells. USe Diagnosis of Rabies IN animals. Use Serodiagnosis of Syphilis 1ry Antibody 2ry Antibody ويه Antibody وه CELL CeLL Direct indirect (ELISA) Detect Antigen or. antibodies Label :- Enzyme. Detect Antibody Detectigen Substate 088 Jubstrate ty Antibody Ag Direct Jubstrate Enzyme 2ny Antaly Ag Any Ratibaly Indirect Enzyme Detediow Ag Capture Antibody Sandwich Radio Jio inmuno assay Label: gamma emitting Isotope [Radante indive] Radioactive Antiyen -RIA Y Y 1st Antibody Unlabelled Antigen 2nd Antibody Two Antigens Compate For binding sites T of Antibody Idea Competitive baday of ladalabaded Aintzer and unlabeled antigen to Antibody Direct Coombs test Diagnosis of Fostalis RBC RBC RBC ) Antibodies bound to RACS but do not Cause agghitinesties Anti. Ig [Coombs Reagent] RBC RBC RBC RBCS agglutinate RBC 8612

Interaction Microbiology Note

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