University:
Massachusetts Institute of Technology
Course:
20.440 | Analysis of Biological Networks (BE.440)
Academic year:

2022
Views:

475

Pages:

9
Author:

Alejandro Callahan

BE.440 Essigmann Last Day: Transcription (Front End) a) promoter architecture b) how pol. finds promoter c) elongation of transcript d) termination e) specialized endings (capping, poly A) f) translation emphasized fact that these segments cause 10^X variation in constitutive expression. Effectors: other way to regulate. Good for responding to changes in the environment. Paradigm of α and ℗ Regulation: (100 foot level) reverse alphabetical order Chemistry: Lactose Gal (β1→4) + Gle Promoter/operator region (100 mm level) [Diagram: A schematic representation of the Lac operon is shown. It includes: ] I: Repressor protein P: Promoter O: Operator Z, Y, A: Genes for β-galactosidase, permease, and transacetylase [Arrows indicate the direction of transcription and the binding of the repressor to the operator.] CRP: Catabolite Repressor Protein (CRP) cAMP: Cyclic AMP [Textual annotations on the diagram explain:] Positive Regulation: Binding of CRP to the operator is needed to ensure that the cell will use glucose first if available. Negative Regulation: The repressor protein (I) binds to the operator and prevents transcription. Kd = 10^-10 M allolact: Allolactose (inducer) binds to the repressor, causing it to release from the operator. [Diagram: A simplified representation of the lactose operon is shown, highlighting the role of allolactose and CRP in regulating gene expression.]

Decoding Information II (Translation)

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