University:
Purdue University
Course:
CHM 62000 | Spectrochemical Instrumentation
Academic year:

2021
Views:

423

Pages:

32
Author:

Isis Welch

Operating Asylum Cypher AFM Appropriate Samples and Sample Prep Samples must fit on a 15mm diameter disk and cannot be more than about 1cm thick. Samples must be flat since the microscope cannot measure features taller than 2.5µm. The maximum area that can be imaged with the microscope is 10µm by 10µm. Stuff to Buy: Description Magnetic disks for sample mounting Adhesive tabs 10mm mica disks Tweezers for handling probes Vendor Ted Pella Ted Pella Ted Pella Ted Pella Page 1 of 32 Part no. 16218 16079 50 5374-NM Page 2 of 32 Page 3 of 32 Page 4 of 32 Page 5 of 32 Page 6 of 32 Page 7 of 32 Page 8 of 32 Page 9 of 32 Page 10 of 32 Page 11 of 32 Engaging the Cantilever on the Sample 0. Log in to the computer and start MFP3D template.pxt software Page 12 of 32 Page 13 of 32 Page 14 of 32 Page 15 of 32 Page 16 of 32 Page 17 of 32 Page 18 of 32 Page 19 of 32 Page 20 of 32 Page 21 of 32 Page 22 of 32 Page 23 of 32 Page 24 of 32 Page 25 of 32 Page 26 of 32 Page 27 of 32 Page 28 of 32 Page 29 of 32 Page 30 of 32 Data Analysis 1. To open saved data, on the menu bar click AFM Analysis  Browse Saved Data and double click the file to open. The appearance of the data can be changed using the display window. 2. On the display window click M to open the Modify panel a. On the Mask tab, click in the histogram and drag the blue line to create a mask that highlights the features but not the background b. On the Flatten tab, check the box that says Offset Uses: Mask. Experiment by flattening with the different polynomial orders to improve the appearance of the data. The Undo Flatten button can be used to undo the previous flatten operation. c. On the display window click Commands  Export to Layout to add the image to the layout for saving/printing. You can also click Commands  Export Image as Tif to create a tif image file of the data. 3. On the display window click A to open the Analyze panel a. On the Section tab click Draw. Click and drag on the image to draw a line through features of interest. Drag the two cursors on the Section plot and they will appear along the line on the image. Drag the cursors to features of interest to make measurements such as the distance between the two features. You can copy the section plot and associated data to a layout for saving/printing b. For this step to work you must have a mask active as described in 2a above. On the Particle Analysis tab click Analyze Particles. Click Find Particles button to find particles that meet certain criteria. Particle Stats button gives count and stats of located particles. 4. Click menu bar  File  Save Experiment As to save your acquisition settings and analysis operations 5. Click menu bar  File  Print Layout to save your layout as a pdf When you are finished 1. If you are scanning, follow the procedure under “Stopping Imaging” above. 2. Click Master Panel  Main tab  Withdraw 3. Click Engage Panel  Approach tab  Move to Pre-Engage to remove the tip from the surface Page 31 of 32 4. While looking into the microscope to monitor the distance between the tip and the sample, rotate the engage control knob clockwise to raise the tip several mm above the sample surface. Caution: If you turn the engage control knob the wrong direction, you will crash the probe into the sample! 5. Open the microscope and slide the scanner forward enough that you can remove the cantilever holder by loosening the two screws using the screwdriver stored inside the microscope and turning the cantilever holder counter clockwise. Caution: If you slide the scanner too far forward it will fall out of the microscope! 6. Rotate the engage control knob counter clockwise to raise the sample stage to its highest position. 7. Remove your sample from the stage. 8. Rotate the engage control knob clockwise to lower the stage to its lowest position. Caution: If you don’t lower the stage far enough, the stage will interfere with the cantilever holder during the next step. 9. Remove your probe from the cantilever holder and replace the cantilever holder on the scanner. 10. Slide the scanner back into the microscope and close the door. 11. Exit the software and log out of the PC Page 32 of 32