Digestive Lab Document Answer Key

Digestive Lab Document Professor Provenzano First, start the gastric digestion experiment. See instructions below. The digestion of proteins in the stomach is accomplished by gastric juices containing hydrochloric acid and the enzyme of pepsin. Use a marking pencil to label 3 test tubes 1-3 and with your groups initials. To test tube 1, add 5ml of pepsin solution To test tube 2, add 5ml of hydrochloric acid To test tube 3 add 5ml of gastric juice (pepsin and hydrochloric acid) Place one scoop of of albumin (a protein found in egg whites and blood) in each test tube. Stir each test tube (be sure to thoroughly clean the glass stirring rod between each test tube). Now place your test tubes into the water bath which has been set to 98.6 degrees Fahrenheit for 45 minutes. Once the time is complete remove the test tubes. Add 2-3 drops of biuret reagent to each test tube and stir. Biuret reagent reacts with proteins when they breakdown and turns pink in color. Record any color change. After completing the experiment, answer the questions below: HCl activates pepsin (acidity activates pepsin) and pepsin breaks down proteins In which test tube did protein digestion occur (or should have occurred) ? 3 Why was the water bath set to 98.6 degrees F? Body temp Which cells secrete pepsin? Chief cells Why is pepsin considered an enzyme? It acts as a catalyst to speed up protein breakdown into peptides Which cells secrete hydrochloric acid (HCl)? Parietal cells Why is the stomach normally very acidic? HCl helps break down proteins Label the teeth below: Central incisors Lateral incisors Canine (cuspid) 1 st premolar 2 nd premolar 1 st molar 2 nd molar 3 rd molar (wisdom tooth) Label the structures in the oral cavity below: Hard palate Soft palate Palatoglossal arch Palatopharyngeal arch Palatine tonsils lingual frenulum vestibule uvula fauces Label the salivary glands and ducts below: Sublingual ducts Sublingual salivary glands Submandibular ducts Submandibular salivary glands Parotid ducts Parotid salivary glands Get the Somso molar tooth model and label the structures with their corresponding numbers below: Structure: Number on Model: Crown 2 Root 4 Enamel 5 Dentin 6 Cement 7 Dental pulp 8 Now get the Somso stomach model and label the structures below with their corresponding numbers on the model: Structure: Number on Model: Cardia 1 Fundus 2 Lesser curvature 3 Greater curvature 4 Pylorus 5 Longitudinal muscles 7 Circular muscles 8 Oblique muscles 9 Rugae 18 Label the parts of the intestines below: Duodenum Jejunum Ileum Large intestine (colon) Rectum Label the parts of the biliary tree below: Right hepatic duct Gallbladder Common bile duct Cystic duct Common hepatic duct Left hepatic duct Label the parts of the hepatopancreatic ampulla below: A. pancreatic duct B. common bile duct C. duodenal ampulla (ampulla of Vater or hepatopancreatic ampulla) D. hepatopancreatic sphincter (Sphincter of Oddi) E. Duodenal papilla Label the lobes and structures below: Right lobe Left lobe Falciform ligament Gallbladder Label the Spleen and pancreas below: Word bank: common bile duct, hepatic artery, hepatic portal vein, pancreatic duct 1: Pancreatic duct 3: Common bile duct 5: Hepatic portal vein 9: Hepatic artery Label the large intestine (colon) below: Right colic (hepatic) flexure Transverse colon Ascending colon Ileocecal valve Cecum Appendix Ileum Rectum Sigmoid colon Taenia coli Haustra Descending colon Left colic (splenic) flexure Draw out what you see in the liver slide and label the central vein, hepatocytes and sinusoids. Draw out what you see in the intestine slide and label the three layers: mucosa, submucosa, muscularis Now, complete the mini practical set up in the lab: Name the structure: Tongue Name the space: Mouth or oral cavity (buccal cavity) Name the structure: Uvula Name the region: Oropharynx Name the structure: Gallbladder Name the structure: cystic duct Name the structure: Esophagus Name the curve: Greater curvature Name the structure: Pyloric sphincter Name the ridge: Rugae Name the region: Duodenum Name the structure: Pancreas Name the region: Cecum Name the structure: Ileocecal valve Name the region: Jejunum Name the region: Rectum Name the structure: Parotid salivary glands Name the specific area: Right lobe of liver Name the region: Transverse colon Name this bend: Left colic (splenic) flexure Name the tooth: Central incisor Name the tooth: First molar Name the layer: Dentin Name the layer: Mucosa Name the layer: Submucosa Name the layer: Muscularis