Sheep Brain Anatomy Lab Manual

Sheep Brain Anatomy Lab Manual I. Introduction The brain of the sheep is useful for study because its anatomy is similar to human brain anatomy. Although exact proportions (and names) sometimes differ, every structure you will identify in the sheep brain corresponds to a homologous structure, usually with the same name, in humans. You and your partner will share one brain for initial study, and a second brain for review later. After studying dorsal, ventral, and mid-saggital features, you will cut one of your half-brains in horizontal sections, and the other half-brain in frontal sections. You will repeat the entire procedure on the second brain for review. Proceed slowly and carefully in your dissection so as not to destroy structures prematurely. Save your horizontal and frontal sections in preservative for later review. A list of all structures you may be asked to identify on the lab practical is at the end of this lab handout, and those structures are underlined the first time they are mentioned in any paragraph in the text below. II. Terminology Be sure you clearly understand the following terms of orientation in reference to the central nervous system: dorsal, ventral, anterior, posterior, superior, inferior, proximal, distal, lateral, medial, rostral, caudal, cranial. Also be sure you know the type of brain section indicated by (1) sagittal or longitudinal, and the special case of midsagittal; (2) coronal or frontal, (3) horizontal and (4) transverse/oblique. Confusion may arise with these orientational terms because humans and other primates walk on their hindlegs, with the spinal cord entering under the brain, while other mammals walk on four legs with the spinal cord entering at the back of the brain. The names of anatomical structures began as descriptive Latin terms. Some authors use the Latin terminology, while others use the anglicized form of the Latin names (e. g. the Latin corpus trapezoidem becomes the trapezoid body; capsula interna becomes the internal capsule). (To complicate further the terminology picture, the same anatomical structure may have more than one distinct name (e.g., Ammon's horn for hippocampus; crus cerebri for cerebral peduncles), and it may not always be generally agreed how inclusive or exclusive a given name is. Major CNS structures such as the thalamus contain smaller individual nuclei, i.e. groups of cell bodies. (Outside the CNS such groups of neuron cell bodies are called “ganglia;” the singular is “ganglion.) Nomenclature of nuclei within the brain often follows a logical, directional pattern, e.g., ventral posterior lateral (VPL) thalamic nucleus which would be the ventrally placed nucleus at the rear of the lateral part of the thalamus. Other names combine position and description, e.g., lateral geniculate (geniculate: diminutive of knee, thus, little knee). Others are purely descripitive, e.g., nucleus gracilis, (slender nucleus), hippocampus (sea horse), pulvinar (a couch). The description can be quite fanciful! Terminology for fiber tracts often follows a “from-to” pattern, e.g., spinothalamic tract 1 (running from the spinal cord to the thalamus), corticospinal (cortex to spinal cord), spinocerebellar, reticulospinal (from reticular formation to spinal cord), tectospinal (from tectum to spinal cord), and mammillothalamic (from mammillary bodies to the thalamus). Fiber tracts go by several names other than tract, which you should recognize. A tract is often called a fasciculus (little bundle) or a stria (narrow band). A funiculus (little cord) is usually larger and less functionally specific than a fasciculus or a stria. Attention to various prefixes will often clarify a seemingly complex name, as in periaqueductal gray, (around the aqueduct), interpeduncular cistern (between the peduncles), parafascularis (beside the fasciculus), pretectal area (in front of the tectum), retrosplenial (behind the splenium). III. External Features of the Brain Of the three coverings or meninges of the brain, your specimen will have only the pia mater completely intact. The dura mater has been removed with the skull, except for a fragment at the base of the brain near the pituitary gland. The arachnoid, a rather filmy membrane lying between pia and dura, will be found relatively intact in protected areas. As you proceed in your study of the brain you should carefully clean the pia and blood vessels from the brain. Always do this slowly and as you go so that you will not destroy structures. Be particularly careful not to pull off cranial nerves with the pia. To do so, gently pry the dura mater away a few millimeters and visualive the nerves as they enter and exit the meninges. Then plan your cutting accordingly. You should not expect to preserve all nerves with meninges removal, but do your best. On the whole brain, locate the following major subdivisions of the central nervous system. The myelencephalon, or medulla oblongata, is the most caudal portion of the brain. It may be distinguished from the metencephalon, which is located immediately rostral of it, by the prominent swelling associated with the latter. This swelling is called the pons, which consists of fibers which are passing dorsally to reach a second part of the metencephalon, the cerebellum. Rostral to the metencephalon is the mesencephalon, or midbrain. When this is viewed on the ventral surface of the brain, it is distinguished by two columns, separated by a depression, extending between the metencephalon and the posterior portion of the next brain division, the diencephalon. These columns, the cerebral peduncles, consist of myelinated fibers which travel from the cerebral cortex to the pons and spinal cord. To study all ventral structures, you must remove the pituitary gland. First study its attachment to the brain and then remove it being careful not to tear off the 5th (trigeminal) nerve, the large trunks of which straddle the gland. Try to do as little damage as possible to the stalk of the pituitary. On the dorsal aspect of the midbrain are four elevations, the superior and inferior colliculi, collectively called the corpora quadrigemina. These may be seen if the cerebellum is gently separated from the cerebral hemisphere. Be careful not to tear the connection between the cerebellum and the pons when you are making this observation. Rostral to the mesencephalon is the diencephalon. The portion of the diencephalon visible on the ventral surface of the brain is the hypothalamus. You will see the thalamus, which is another portion 2 of the diencephalon, when you view the midsagittal section, as well as in horizontal and frontal sections. The diencephalon is bordered posteriorly by a well-marked prominence, the mammillary body, which in humans is divided by a longitudinal crease into two separate mammillary bodies. Anteriorly, the optic chiasm marks the rostral border of the diencephalon. The remainder of the brain, i. e., the cerebral hemispheres, constitute the telencephalon. The cerebral hemispheres include the cerebral cortex (i.e. the outer layer of gray matter; cortex = bark of a tree), the underlying white matter or fibers, various nuclei within the hemispheres, and the rhinencephalon ("smell brain"). The rhinencephalon includes all those structures directly related to olfaction (olfactory bulb, olfactory nerve, and olfactory tract), and others not related to olfaction that are structurally associated, and which you will see later (septal area, hippocampus, hippocampal gyrus). These five encephalic divisions— metencephalon, myelencephalon, mesencephalon, diencephalon, and telencephalon— together with the spinal cord, make up the central nerous system. Within these five major subdivisions, locate the following structures: A. Myelencephalon. On the ventral surface locate the pyramids on either side of the mid-line. These bumps are made up of fiber tracts coming from the primary motor gyrus of the cerebral cortex and passing to the spinal cord. In the mesencephalon the same fibers constitute the cerebral peduncles. In the region of the caudal medulla in humans, 80% to 90% of these pyramidal fibers cross from one side to the other, i.e. they decussate. Thus, a lesion in this tract above the level of the medulla would result in motor problems on the contralateral side of the body. B. Metencephalon. Locate the brachium pontis (also called the middle cerebellar peduncle) which is the bundle of fibers responsible for the enlarged size of this portion of the brain. Fibers of the brachium pontis arise from cells in the pons and pass to the cerebellum on the other side. On the ventral surface you may be able to locate the 6th, 7th, and 8th nerves (though these are not always clearly visible, and are optional in this dissection), and more rostrally the 5th (trigeminal) cranial nerve. At the lateral junction of the medulla and cerebellum you will notice a little tuft of choroid plexus which projects from a small aperture in the pial membrane. This is the location of the foramen of Lushka. It is one of the means by which the cerebrospinal fluid passes from the fourth ventricle (seen more clearly in later saggital section) into the sub-arachnoid space between the cerebellum and medulla. This enlargement of the sub-arachnoid space is the cisterna magna. On the dorsal surface of the metencephalon note the cerebellum and identify the cisterna magna. There is also a medial foramen in this region serving as a communication between the fourth ventricle and the cisterna magna. It is called the foramen of Magendie. This foramen will be better seen after sagittal section. The cerebellum, like the cerebral cortex, has convolutions on its outer surface. In the cerebellum they are called folia. The cerebellum is composed of an unpaired median portion, the vermis cerebelli, and two lateral masses, the cerebellar hemispheres. 3 C. Mesencephalon. On the ventral surface of the mesencephalon, locate the cerebral peduncles and the interpeduncular cistern, another reservoir of cerebrospinal fluid. Dorsally you may see once again the superior and inferior colliculi. The superior colliculi are much larger than the inferior, and the difference is greater in sheep than in human. Also note the superior cistern (Ambiens) between the rostro-ventral portion of the cerebellum and the colliculi. (A knowledge of the normal anatomy of these cisternae as out-lined in air or contrast media in radiographic studies is often helpful in the diagnosis of tumors or other masses in the CNS.) In this region of the brain locate the 3rd (oculomotor) and 4th (trochlear) cranial nerves. The trochlear nerve is tiny and may not be visible on your brain. The trochlear nerve is unique among cranial nerves in that it exits dorsally from the brain stem. D. Diencephalon. On the ventral surface of this region locate the optic tract and optic nerve and note again the optic chiasm and the mammillary body. Between these structures you will notice the stalk of the pituitary gland (hypophysis). This stalk, the infundibulum, arises from a small mound or elevation on the ventral surface of the hypothalamus called the tuber cinereum or median eminence. Dorsally, you should be able to see the pineal body (also known as the epiphysis) by sighting above and between the superior colliculi. The myelencephalon, metencephalon, mesencephalon, and diencephalon are spoken of collectively as the brain stem. E. Telencephalon. The telencephalon consists of the cerebral cortex and the basal ganglia. The latter are deep structures not visible in the undissected brain. During fetal growth, the pattern of cell proliferation and migration results in the surface of the cerebral cortex developing folds or convolutions called gyri (singular: “gyrus”), separated by grooves called sulci (singular sulcus). Major divisions, consisting of several or many gyri, are called lobes; deep sulci separating one lobe from an adjacent one are called fissures. The telencephalon, particularly the cerebral cortex, is the area of the brain that varies most among mammals. The human telencephalon is greatly enlarged, burying many of the structures seen on the surface in the sheep brain. The brain stem, on the other hand, is relatively constant throughout the mammalian class. Now consider for a moment the cingulate and subcallosal gyri: how would you categorize these ? Discuss amongst your lab-mates. Note the cerebral cortex with its many gyri and sulci or fissures. On the dorsal surface the medial longitudinal fissure separates the two hemispheres. The separation is complete at the anterior end (frontal pole) and posterior end (occipital pole). If you carefully separate the two hemispheres at the longitudinal fissure, you will see the dorsal surface of the corpus callosum. The callosal sulcus separates the corpus callosum from the adjacent mid-line cortex, the cingulate gyrus. The cingulate sulcus separates the cingulate gyrus from the more superior gyri. The medial surface of the cortex will be better visualized and more carefully studied after sagittal section of the brain. For now, see what you can in the narrow space between the intact hemispheres. 4 The cruciate fissure (labeled ansate sulcus in your photo atlas) is known in the human brain as the fissure of Rolando or central sulcus, and intersects the medial longitudinal fissure to mark off the anterior third of the cortex. The gyrus immediately anterior to the cruciate fissure is the precentral gyrus (labeled precoronal gyrus in your photo atlas), the primary motor area of the cortex. Immediately posterior to the cruciate fissure is the postcentral gyrus, the primary somato-sensory area of the cortex. Intersecting the cruciate fissure at right angles and extending anteriorly is the superior frontal sulcus. On the lateral surface, the insula or Island of Riel is the floor of a slight depression of the cortical surface. Sensory and motor representation of the viscera are located here along with some of the cortical representation for taste. Arching upward in a dorsal-anterior direction from the insula is the Sylvian fissure, also called the lateral fissure (Sylvian) or sulcus. The rhinal fissure extends horizontally on the line where the lateral and ventral surfaces meet. It separates the neocortex of the cerebral hemispheres from the rhinecephalon, the phylogenetically older cortex associated with olfactory sense. In humans the development of the neocortex has completely buried the insula and the rhinal fissure and associated rhinencephalic structures. Many other sulci and gyri are visible on the surface of the sheep brain, but they are neither constant in position for the sheep nor necessarily homologous to any similar structure in other species. After you have located the primary fissures you can delineate the lobes of the cortex. The portion of the cortex anterior to the cruciate fissure is the frontal lobe. The most posterior part of the cortex is the occipital lobe. Between the occipital lobe and cruciate fissure lies the parietal lobe. There is no clear line of demarcation between parietal and occipital lobes. Posterior to the Sylvian fissure and dorsal to the rhinal fissure lies the temporal lobe. On the ventral surface the olfactory bulb is easily identified as a padlike structure placed against the frontal pole. It lies in a depression, the olfactory sulcus. The ventral cortex lateral to the sulcus is the orbital gyrus. Cortex medial to the olfactory sulcus is the gyrus rectus. The lateral and medial olfactory striae course backward from the olfactory bulb. Between the olfactory striae and the optic tract and chiasm is the anterior perforated substance, which receives fibers from the medial and lateral olfactory tract and relays to the hypothalamus. Immediately in front of the optic tracts lie a band of lighter color, the Diagonal Band of Broca, which carries fibers from the septal area to the amygdala. Follovnng the lateral olfactory stria posteriorly, the rather large lobe encountered is the hippocampal gyrus, not to be confused with the hippocampus itself, which lies deep inside the gyrus and will be studied later. The hippocampal gyrus, or sometimes its anterior portion combined with the lateral olfactory striae, is sometimes called the pyriform lobe or area. The posterior part of the hippocampal gyrus is called the entorhinal cortex. An indistinct medial bulge on the hippocampal gyrus in the vicinity of the optic tract is created by the underlying amygdala, which will become visible when you make sections of the brain. IV. Blood supply Identify the Circle of Willis, which is made up of the major arteries surrounding the 5 optic chiasm and pituitary. The anterior communicating artery completes the anterior portion of the circle, while the posterior portion is formed by the union of the posterior communicating arteries. This circle allows communications between the carotid and vertebral or basilar supplies to the brain, and provides for a collateral circulation in case one of the tributary vessels is occluded. The primary difference between the blood supply to the sheep brain and that to the human brain is that a single basilar artery enters the cranium in the sheep, while in the human two vertebral arteries enter the cranium and then join to form the basilar artery. Thus the arteries entering the cranium are the internal carotids which branch from the common carotids in the neck, and the vertebrals (or basilar) which branch from the subclavian arteries in the neck. The venous return to the heart runs in the dura and thus has been removed from your specimen. The venous return is collected in sinuses and returns to the heart via the jugular vein. Your knowledge of the blood supply of the brain will aid in your understanding of methods used to "fix" the brain for experimental or histological purposes. Animals' brains can be perfused with the fixing solutions by introducing the solutions (normal saline and formalin) into the common carotids in the neck and draining the blood from the external jugular vein in the neck. In this way the fixative perfuses the entire brain. V. Mid-sagittal Section of the Brain Now cut the brain in the mid-sagittal plane. The instructor will assist you in doing this properly. Many of the structures you have already identified will now appear in a new perspective. Some of these structures will appear in the following instructions, but you should be sure to locate all previously mentioned structures which now appear in the midline. Before making the sagittal section your brain should be cleaned of vessels and pia mater. Starting at the caudal end of the myelencephalon, note the central canal which extends caudally into the spinal cord and rostrally into the fourth ventricle. You should now be better able to see the foramen of Magendie, communicating between the fourth ventricle and cisterna magna. In the metencephalon note the fourth ventricle lying beneath the cerebellum. Examining the median view of the cerebellum, you will see the white matter of the core as it projects treelike—hence the name arbor vitae—into the folia of the cerebellum. You can now more clearly see the deep fissures of the cerebellum which divide it into lobes. The cerebral aqueduct, or aqueduct of Sylvius, or runs from the rostral portion of the metencephalon through the mesencephalon to connect the fourth ventricle with the third ventricle of the diencephalon. The roof of the aqueduct is made up of the lamina quadrigemina with its superimposed quadrigeminal bodies. The portion of the midbrain above the aqueduct, including the superior and inferior colliculi, is called the tectum; the portion below the aqueduct and above the cerebral peduncles is called the tegmentum. 6 Moving rostrally, we come to the diencephalon. First, identify the thalamus with its large central mass, the massa intermedia, which extends across the midline in most specimens. The fluid-filled space between the hemispheres and surrounding the massa intermedia is the third ventricle. Ventral to the thalamus lays the hypothalamus. There is no clear separation between thalamus and hypothalamus seen in sagittal section. The hypothalamus integrates visceral-somatic activity and controls the secretion of hormones from the pituitary gland. Part of the control of feeding behavior, drinking, sexual behavior, temperature regulation, and rage responses, among other functions, are carried out by the hypothalamus. The thalamus is a relay and integrating station for sensory pathways ascending on the way to the cerebral cortex. The thalamus also has diverse other functions. Having diffuse reciprocal connections with the cerebral cortex, it plays a part in attention and arousal mechanisms. Many structures referred to in this paragraph will be seen in more detail when you do frontal and horizontal sections. Part of the dorsal and anterior limit of the thalamus is formed by the fornix (meaning arch), a bundle of fibers arising below the posterior portion of the corpus callosum and arching downward to dive below the surface in route to the mammillary bodies. The fornix arises from a band of fibers, the fimbria, which runs along the medial surface of the hippocampus. (Both fimbria.and hippocampus will be seen later.) One fornix is connected to the other by transverse fibers called the commissure of the fornix, or hippocampal commissure. The portion of the fornix where the two are connected is called the body of the fornix while the separate downward arching part is called the column of the fornix. The fornix carries fibers from the hippocampus to the mammillary bodies (via the postcommissural fornix) and septal nuclei (via the precommissural fornix) and fibers.from septal nuclei to hippocapmus. Near the anterior arch of the fornix you will note a small round bundle of fibers, the anterior commissure, which connects the olfactory bulbs, pyriform area and amygdala of the two hemispheres. Completing the anterior border of the diencephalon is the lamina terminalis, which forms part of the anterior wall of the third ventricle. At the posterior end of the thalamus you will again see the pineal body. Immediately anterior to the pineal body is the habenula, a thalamic nucleus thought to be associated with olfacto-somatic correlation. Below the pineal body and anterior to the colliculi is the posterior commissure, another bundle of fibers connecting the two hemispheres. It forms the border between diencephalon and mesencephalon and is just dorsal to the point where the cerebral aqueduct becomes continuous with the third ventricle. Just below the fornix anteriorly, note the interventricular foramen or foramen of Monro, which communicates between the lateral ventricle (to be studied later) and the third ventricle. Now examine the corpus callosum, the large band of fibers which serves to connect the two hemispheres. The sharply bent anterior portion is called the genu (meaning knee). As the genu turns under, it narrows into the rostrum (meaning beak) of the corpus callosum. The posterior end of the corpus callosum is called the splenium. The thin sheet of tissue running between the corpus callosum and fornix is the septum pellucidum. It forms the medial wall of the lateral ventricle, and part of the lateral wall of the third ventricle. Depending on how well your incision is placed, it may already be open on one side and not the other. If both sides are 7 covered, pierce and open this wall with small forceps and view the ventricle within. Turning now to the cerebral cortex, it is questionable whether all of the gyri and sulci seen on the medial surface are analogous to those of man. The one primary and constant sulcus of the midline cortex is the cingulate sulcus. Between this sulcus and the corpus callosum is the cingulate gyrus. The callosal sulcus lies between cingulate cortex and corpus callosum. The portion of the cingulate gyrus around and behind the splenium of the corpus callosum is called retrosplenial cortex. The cortex in front of and below the genu of the corpus callosum is the subcallosal gyrus. Cortex below this is called the parolfactory gyrus. This cortex below the rostrum of the corpus callosum as well as some more laterally placed nuclei is the septal area. The sub-callosal and parolfactory gyri are included in the term septal area. You should recognize that the cingulate gyrus extending around the posterior end of the corpus callosum becomes continuous with the hippocampal gyrus, which you saw on the ventral surface. As dissection continues, you will be able to better visualize this continuity. The cingulate gyrus together with its continuation as the hippocampal gyrus is often called the limbic lobe as it tends to make a border around the upper end of the brain stem (limbic = border). The term limbic lobe is, however, often used synonymously with limbic system which includes the hippocampus, hippocampal gyrus, amygdala, septal area,. cingulate gyrus and.often the olfactory structures as well. Thus the term “limbic lobe” comes to include most of the rhinencephalon as well as additional structures thought to be functionally related. As you can see, the terminology is not universally agreed upon here. Whatever the terminology, these structures are closely connected anatomically via the fornix, stria terminalis, stria medullaris, anterior commissure, diagonal band of Broca, and the medial forebrain bundle. VI. Deep Structures of the Brain 1. Cerebellum, fourth ventricle, and dorsal view of the brain stem. Remove the cerebellum by carefully making successive horizontal sections through the cerebellum. As you make these sections, note how the white central core projects into the lobules and folia. As you continue these horizontal sections, and by reflecting the cerebellum in various directions as you progress, you should note once again the middle cerebellar peduncle (brachium pontis) as it enters the cerebellum as a continuation of the transverse fibers of the pons carrying primarily pontocerebellar fibers. Entering the cerebellum medially to the brachium pontis and extending upward from the cerebellum to end just .beneath the inferior colliculus is the superior cerebellar peduncle (brachium conjunctivum) which connects midbrain structures with the cerebellum. The inferior cerebellar peduncle (restiform body) enters the cerebellum caudally carrying fibers to and from the medulla oblongata. All afferent and efferent fibers of the cerebellum must pass through one of these three peduncles. You can now also identify the dorsal cochlear nucleus, a compact, crescent-shaped maas of gray matter encircling the restiform body at the point where the latter turns dorsal-ward to enter the cerebellum. The 8th (auditory-vestibular) nerve will be seen associated with this nucleus at its lateral extent. The dorsal cochlear nucleus (along with the ventral cochlear 8 nucleus, which is not visible on the surface) is the first site of synapse of the 8th nerve fibers from the cochlea. By gently lifting the cerebral hemispheres you can see the posterior.portion of the thalamus above the superior colliculus. The medial portion above the pineal body is the habenula which you have seen on the medial surface. Lateral to the habenula is the pulvinar, a functional puzzle which has afferent and efferent cortical connections. From the inferior colliculus you can follow a distinct band of fibers, the brachium of the inferior colliculus, anterolaterally to a prominent swelling, the medial geniculate, the thalamic relay nucleus for auditory fibers. The primary auditory pathway passes from the cochlear nucleus (which you have seen previously) to the inferior colliculus via a tract called the lateral lemniscus, then to the medial geniculate via the brachium of the inferior colliculus. From the medial geniculate the fibers are relayed to the primary auditory cortex in the temporal lobe via the sublenticular portion of the internal capsule (may not yet be visible). This pathway crosses at many levels prior to the geniculate, so that hearing is well bilaterally represented. Above the medial geniculate the large swelling is the lateral geniculate; by lifting the hippocampal gyrus you may trace the optic tract to the lateral geniculate. This nucleus is the thalamic receiving area for vision, and the fibers are relayed from it via the geniculocalcarine tract (or optic radiation) through the sublenticular portion of the internal capsule to the primary visual cortex in the occipital lobe. 2. Fornix, mammillothalamic tract, and Papez' circuit. Return to the medial aspect of the half-brain. With a sharp scalpel, remove a slice approximately one millimeter thick of the thalamus anterior to the massa intermedia and posterior to the anterior commissure, by making a 1-mm deep cut parallel to the medial surface. Identify the column of the fornix as it runs to the mammillary body. At about the same depth but posterior to the fornix at about the center of the mammillary body, you should find another distinct band of fibers, the mammillothalamic tract. This tract runs from the mammillary body to the anterior thalamus and is part of Papez' circuit of the limbic system. Fibers from the anterior thalamus are projected to the cingulate gyrus, which projects fibers to the hippocampus, which closes the circuit by projecting back to the mammillary body via the fornix. This circuit typifies the close interrelations and feed-back possibilities characteristic of the various structures of the limbic system. 3. Horizontal section opening the lateral ventricle. Tear away the septum pellucidum and examine the lateral ventricle. Note that the corpus callosum forms the roof of a large part of the cavity. Identify the head of the caudate nucleus in the anterior floor of the ventricle. In the medial and posterior floor of the ventricle identify the hippocampus and the fimbria of the hippocampus running along its lateral and anterior edge. Note the position of these structures now, realizing that you will be able to see 9 them more clearly when you have opened the roof of the ventricle by horizontal section. With a scalpel make successive, thin horizontal sections through the cerebral cortex until you open the roof of the ventricle. Proceed slowly so as not to destroy structures before you examine them. As you progress with these sections note the white matter as it projects into the gyri of the cerebral cortex. This white matter, the corona radiata, is composed of sensory fibers ascending from the thalamus and motor fibers descending to lower structures. As you proceed with horizontal sections you will be able to follow these motor fibers downward into the internal capsule, cerebral peduncles, and pyramids of the medulla. The radiations of the corpus callosum are also mingled with this white matter. Continue this process of successive sectioning until you have opened the roof of the lateral ventricle and cut through the dorsal part of the corpus callosum on a plane parallel to the callosum. Now again examine the ventricle. The head of the caudate in the anterior floor of the ventricle plunges downward in the anterior horn of the lateral ventricle until it becomes continuous with the anterior perforated substance. Running with the caudate along its medial edge at its junction with the thalamus is the stria terminalis which carries fibers from amygdala (may not yet be visible) to septal area. In the posterior floor of the ventricle note once again the hippocampus and fimbria (be careful to not make too many cuts or you may not see them). The hippocampus is covered by a thin layer of white matter, the alveus, which becomes continuous with the fimbria which is in turn continuous with the fornix. If you gently scrape a small area of the alveus with a scalpel, you can expose the underlying gray matter of the hippocampus. Note the transverse fibers of the hippocampal commissure. The hippocampus arches downward around the thalamus in a manner similar to the caudate and ends in the hippocampal gyrus posterior and lateral to the amygdala. By making careful, thin frontal sections beginning at the occipital pole you can open the back of the lateral ventricle and see the arch of the hippocampus as it turns downward in the inferior horn of the lateral ventricle. If you keep in mind these overall descriptions of the caudate and hippocampus, their anatomical structure will become clear to you as you continue horizontal sections. 4. Horizontal section through the dorsal thalamus. Continue the horizontal sections until you reach the dorsal surface of the thalamus. This section should be parallel to the plane of the corpus callosum and should just pass through the dorsal surface of the massa intermedia. It should pass above the pineal body posteriorly and just below the genu of the corpus callosum anteriorly. Anteriorly you will see the head of the caudate nucleus in the anterior horn of the lateral ventricle. Proceeding laterally from the head of the caudate, the white matter is the internal capsule. It extends posteriorly in an oblique L-shape. Gray matter lateral to the anterior half of the capsule is the putamen, bounded laterally by a thin white stripe, the external capsule. Lateral to this capsule is a band of gray, the claustrum. Medially across the ventricle from the head of the caudate is the septal area bounded posteriorly by the cut edge of 10 the fornix. Posterior to the head of the caudate and medial to the internal capsule is the central gray mass of the thalamus with the medially placed massa intermedia and habenula, the posteriorly placed pulvinar and lateral to the pulvinar, on the border of the thalamus, the gray of the lateral geniculate should appear surrounded by the white of the optic tract. Posterior to the geniculate is the hippocampus and fimbria running in the posterior and inferior horns of the lateral ventricle. Further in the posterior direction, the cerebellum will appear. 5. Horizontal section through anterior commissure. Continue the horizontal sections until you reach a level through the anterior commissure and the superior colliculus posteriorly. The globus pallidus should now appear between the putamen and internal capsule. The globus pallidus and putamen are called collectively the lentiform nucleus. The caudate nucleus and lentiform nucleus are called collectively the corpus striatum, or simply striatum.. These structures are associated with the extrapyramidal motor system. Note how the anterior commissure swings forward in a Ushape, to interconnect the two olfactory bulbs. 6. Continuing horizontal sections Continue these horizontal sections downward to clarify as many anatomical relationships as possible. Be alert to the fact that you are revealing the internal position of many structures that you have already identified on the surface of the brain. At the level of the inferior colliculus, the medial geniculate will appear along with the associated brachium of the inferior colliculus. Note the relationships of the hippocampus, amygdala, and hippocampal gyrus. The cerebral peduncles will be revealed when your horizontal sections reach a level just above the optic chiasm. The peduncles are, of course, the motor fibers you have been following throughout your horizontal sections; first as the corona radiata, then as the internal capsule, and they continue downward into the medulla as the pyramids. 7. Frontal section through the anterior perforated substance. In this and all other frontal sections recall how your horizontal sections looked at various levels. This will help you identify structures seen frontally and will allow you to develop a three dimensional view of the brain. Start your frontal sections from the frontal pole and progress backward slowly and carefully. As you progress, note how the radiations of the corpus callosum blend into the corona radiata, and how the genu of the corpus callosum (medial portion) forms the medial floor of the lateral ventricle. Continue the sections until you reach a level through the anterior perforated substance just anterior to the optic chiasm and passing to the corpus callosum just posterior to the genu so that the rostrum is separated from the body of the corpus callosum (roughly similar to page 31 of photo atlas). When you reach this level, note the head of the caudate nucleus in the anterior horn of 11 the lateral ventricle. Moving laterally and slightly ventral-ward from the caudate you will encounter the somewhat scattered fibers of the anterior limb of the internal capsule. The internal capsule separates the head of the caudate from the lentiform nucleus. (Only the putamen of the lentiform nucleus is clearly visible at this level (page 32/33 of photo atlas). As you dissect backward, the internal capsule will become a more distinct bundle and will clearly separate the caudate from the lentiform nucleus. The globus pallidus will appear ventromedially to the putamen.) This section shows clearly the source of the collective mass of the caudate and lentiform (corpus striatum = striped body) as the internal capsule stripes the nuclei. Lateral to the putamen, the distinct thin white stripe is the external capsule and the more or less distinct gray stripe lateral to the capsule is the claustrum. The large solid mass of white matter located centrally is the corona radiata, and the radiations of the corpus callosum project into it. Medially on this section you should note the rostrum of the corpus callosum and the septal area beneath it. If the septum pellucidum is still intact you can note its continuity with the septal area. Above the ventricle you will find the body of the corpus callosum and the cingulate gyrus and sulcus. You should also note on this section the lateral olfactory stria, anterior perforated substance, lateral fissure, and rhinal fissure. 8. Frontal section through the optic chiasm. Continue backward until you reach a level passing through the optic chiasm and just anterior to the anterior commissure (around page 33 of atlas). In addition to the structures seen on the previous section, this section will clearly reveal the globus pallidus separated from the putamen by a distinct white stripe, the internal medullary lamina. Medially the preoptic area of the hypothalamus appears just above the optic chiasm. Note the distinct bundle formed by the forward directed fibers of the “U” or “cone” shape of the anterior commissure. Ventrally the diagonal band of Broca will have appeared along with the hippocampal gyrus laterally. This section should show the depth of the lateral fissure and should have just opened the third ventricle above the optic chiasm. 9. Frontal sections in the region of the fornix. 12 As you continue these frontal sections backward you will begin to cut through the fornix (around page 34 of atlas). As your section passes just behind the anterior commissure you will be cutting the fornix twice, dorsally and ventrally. It will appear ventrally as a compact round bundle below the middle of the section. As you progress backward the round bundle of fornix will appear lower and lower until it reaches the mammillary body. Your sections will soon pass behind the lentiform nucleus/putamen and the continuity of the cerebral peduncles with the internal capsule will become more apparent. The gray mass of the thalamus medial to the internal capsule will become more prominent as will also the hypothalamus. 10. Frontal section through the mammillary body. Continue frontal sections until you reach a level just through the anterior part of the mammilIary body. You should have followed the fornix so that you can now recognize it as entering the mammillary body. Dorsal to the fornix you should be able to identify the mammilothamalic tract as a similarly shaped bundle of fibers. The cerebral peduncles will appear clearly on this section along with the upward extension as the internal capsule. Lateral to the peduncles note the optic tract (also view the exterior of the brain). At the junction of the hippocampal gyrus with the internal capsule, the stria terminalis will appear as a compact bundle of fibers. By pulling up the hippocampal gyrus you can see the hippocampus and fimbria which will appear in frontal section when you progress slightly more posteriorly. The gray matter at the ventral tip of the hippocampal gyrus is the amygdala. This section also cuts the fimbria (or fornix, depending upon the exact level and angle of your cut) and the hippocampal commissure dorsally (depending on the cut this structure may still lie more caudal). The thalamus appears at about its greatest extend in this section and you will note the rough demarcation of the thalamus into various nuclei by some more or less distinct white bands of fibers. Medially, on the dorsal surface of the thalamus is the stria medullaris thalami, one of the pathways of the limbic system serving to connect septal area, amygdala, parts of the hypothalamus and other structures with the habenula. The hypothalamus, including the mammillary body, appears ventrally on this section (roughly corresponding to page 35 of the atlas). 11. Frontal section through the habenula. As you progress now you may (depending upon your angle of cut) sever the remaining connections holding the brain stem to the cerebral hemispheres and the brain stem will fall away from the hemispheres. You should try to maintain the integrity of the whole brain until you complete this section (to around page 36/37 of atlas). Also, use external views of the brain to aid localization of structures at this phase. Proceed with frontal section until you reach the anterior part of the habenula and expose both the dorsal and ventral hippocampus. The ventral hippocampus can be easily 13 identified surrounded by the fimbria within the hippocampal gyrus. The dorsal hippocampus appears above the thalamus. On the lateral edge of the thalamus the crescent shaped (or knee shaped; geniculate = little knee) lateral geniculate will appear. Medial to it and separated from it by a thin band of fibers is the medial geniculate. On the dorsomedial surface this section will cut through the habenula and a distinct fiber tract coursing downward from it, the habenulointerpeduncular tract, carrying fibers from the habenula to the interpeduncular nucleus of the midbrain. This is one of the pathways by which limbic system influence, which has reached the habenula via the stria medullaris thalami, may reach the mesencephalic reticular formation. The pulvinar comprises the bulk of the posterior part of the thalamus at this level. 12. Separation of cerebral hemispheres from brain stem. At this point the cerebral hemispheres will have separated from the brain stem. You can now study each separately. In the hemisphere, you can roll the hippocampus out of the posterior and inferior horn of the lateral ventricle and clearly visualize its full extent. With the brain stem thus removed, you can also clearly see the continuity of the hippocampal gyrus with the cingulate gyrus around the splenium of the corpus callosum. This view with the brain stem removed should suggest to you how the term “limbic lobe” came to be applied to the cingulate and hippocampal gyri, as they form a border around the brain stem (limbus = border). These structures, and structures anatomically and functionally related to them, are thus called the limbic system. In the brain stem, the lateral and medial geniculate will become more prominent as you continue backward (around page 37 of atlas). At a point slightly behind these structures, you may be able to see the full extent of the habenulointerpeduncular tract as it courses between the habenula and the interpeduncular nucleus. Slightly further back you should note the crossing fibers of the posterior commissure. When your dissection reaches the superior colliculus, its layered fiber core will appear along with the commissure of the superior colliculus. Ventrally, the brachium of the inferior colliculus will appear clearly. The cerebral aqueduct (aqueduct of Sylvius) is surrounded by gray matter, the periaqueductal gray. Within the core of this level of the brain stem are many fibers of passage which include the fibers you have seen as the cerebral peduncles. With further cuts, you can visualize how these will dive through the pons and appear on the other side as the pyramids. The primary, ascending sensory pathway carrying spinothalamic fibers will appear throughout these levels as the medial lemniscus. Many other more or less distinct fiber tracts course through these brain stem levels connecting various parts of the CNS. The nuclei of the cranial nerves are found throughout the pons and medulla, and you can find their approximate location by noting where the nerve exits on the surface. Many other nuclei are scattered up and down the core of the brain stem from the medulla to the posterior part of the thalamus. These comprise the reticular formation, a loosely defined anatomical and functional system. 14 Your continuing dissection will open the fourth ventricle and expose the cerebellar peduncles and the transverse fibers of the pons. Cutting the cerebellum in this way will emphasize its massive fiber connections with the rest of the brain. Continue the dissection, noting the fibers of passage and the core nuclei throughout the medulla. 15 THE PARTS OF THE BRAIN AND ASSOCIATED NERVES AND CAVITIES Major Parts Subdivisions Cranial Nerves Prosencephalon (forebrain) Telencephalon Cerebral cortex Corpora striata Rhinencephalon I Diencephalon Thalamus Hypothalamus II Cavities Lateral ventricle Rostral part of 3rd ventricle Major part of 3rd ventricle Mesesencephalon (mid-brain) Mesencephalon III, IV Corpora quadrigemina Cerebral peduncles Tegmentum Cerebral aqueduct Rhombencephalon (hind-brain) Metencephalon Cerebellum Pons V, VI, VII, VIII 4th ventricle Myelencephalon Medulla oblongata IX, X, XI, XII 16 The Cranial Nerves You are responsible for nerves I through V; the others are for your information but don’t need to be located and memorized. I. Olfactory; many small fibers arising in olfactory mucuous membrane and ending in olfactory bulb. Sensory. II. Optic; from retina to optic chiasm. Sensory. III. Oculomotor; origin in midbrain; supplies four extraocular muscles. Motor. IV. Trochlear; midbrain; innvervates one extraocular muscle. Motor V. Trigeminal; attached to side of pons; very large. Sensory from muscles of mastication, skin of face and scalp, mucous membrane of mouth and nasal cavity, cornea of the eye, teeth, and dura mater. Motor to muscles of mastication. Both motor and sensory. VI. Abducens; arises from trapezoid body. Supplies one extraocular muscle. Motor VII. Facial; lateral to abducens and just behind trigeminal; motor for muscles of facial expression; sensory from anterior two-thirds of tongue (for taste). Both motor and sensory. VIII. Auditory -vestibular; just behind and lateral to facial nerve; serves senses of hearing and equilibrium. Sensory. IX. Glossopharyngeal; arises together with vagus nerve behind and ventral to auditory nerve. Sensory from posterior third of tongue (for taste), other mouth and throat areas. Motor to a salivary gland. Both motor and sensory. X. Vagus; both sensory and motor for heart, stomach, blood vessels, and viscera. XI. Spinal accessory; runs along lateral surface of medulla and spinal cord receiving fibers along the way. Innervates muscles of neck. Motor. XII. Hypoglossal; arises in lower medulla in several more or less distinct roots: Motor to muscles of tongue. A printable mnemonic (arguably better ones are unprintable here!) is as follows: On Old Olympus' Towering Top A Finn And German Viewed Some Hops. 17