Gene cloning
manipulation of DNA to produce many copies of a desired gene sequence
the cloned gene is transferred to a host organism
the host organism then makes multiple copies more readily available for research and others
Procedure:
fragment of DNA, containing the gene to be cloned, is cut by enzymes & inserted into a circular DNA molecule (vector) "recombinant DNA molecule"
the vector transports the gene into a host cell (bacteria)
within the host cell the vector multiplies producing numerous identical copies not only of itself but also of the1 gene that it carries
the vector must be cut with the same enzyme so that it can be easily bind to the DNA of interest
Therapeutic cloning
process of producing genetically identical cells which can then treat various diseases
the cloned cell is used to grow new tissues or organs
Reproductive cloning
process of producing genetically identical organism
method not really successful e.g. 1-6% cloned organisms are born
from those that are born only a small percentage of them scientist explore possibilities to restore endangered species
Therapeutic cloning & stem cells
Stem cells: undifferentiated cells that can reproduce indefinitely & differentiate into specialized cells
totipotent stem cells: give rise to any type of cell
pluripotent stem cells: can produce only certain types of cells
Biotechnological Technologies: Transgenic Organisms
organisms whose genetic material contains DNA from various species
genetically modified organisms
foreign DNA must be inserted into the organism in order for this to take place
plants are modified to increase resistance to insects, pests or viruses or herbicides
transgenic animals are used to produce medical protein