University:
Brigham Young University
Course:
MCB 3020 | General Microbiology
Academic year:

2021
Views:

35

Pages:

4
Author:

NebulaNomad123

Replication in Eukaryotes Three step process - Initiation 2. Elongation 3. Termination Initiation of Replication It is the first step in eukaryotic replication most of the proteins combines to form Pre-Replicative Complex (Pre-RC) * Involved proteins → Origin recognition complex Cell division cycle 6 (Cdc6) Chromatin din licensing. (Cdt-1) and DNA Replicatom-factor 1 → Minichromosome Maintenance Protein Complex (Mem 2-7) ORC binds in the Ori-c Site of the DNA Recruits the Cdc 6 Cdc 6 Binds with DRC in ATP dependent mannen Code 6 recruits the Cdt-1 Cdt-1 is required for licensing the Chromatin in for Replication Cdl I binds with Cterminous of Cde 6 Finally binding all three proteins recruit Mem G1 Replication Initiated SDNA synthe sis Two daughter Copies ans Produced 62 Repaй Mech ranisms in which nobar These followin -9 steps occurs In G1 phase of cell cycle GINS are essential for inkraction of mom and Cda 45 at Dri-c' site during initiation. * GINS Complex is composed of four small proteins namely →S1d5 (Code 105) Psf 1 Cede101) →PSF2 (edc102) →PS/3 (Cdc103) 5-1-2-3 9o-ichien san Jaapanese nung AD LECHVTOСА ДЕНКУДЛИ * At the onset of Sphase, the pre-replicative complex onust be activated by two to form an S-phase-specific kinase in order initation complex at an origin of replication * One kinase is the Cdcf - Dbf 4 Kinase called Of dependent kinase (DDK) and the other is cyclin-dependent Kinase (CDK). *The CDK-dependent phosphorylation of CDe 6 has bee considered to be required for entry into the S-phase * DDK targets the Mem complex, and its phosphorylation leads to the possible activation of mem helicase activity. * Minichromosome Maintenance Complex has helicase Function of MCM Complex betivity and inactivation of any of the six protein will prevent the progress of formation of replication fork *It also has ATPase activity. A mulation at any suret the Mem protein complex will reduced converted At binding sites. * Mem complex is a hexames with Mem B, momt, Mem2, Pem6, Mem 4, Mem5 Initiation Complex * It is the 2nd Stage in DNA vseplication whese the Pre- replicative complex is converted into Initiation complex * Invotred proteins →Cell Division Cycle 45 (Cde45) →GINS T Cyclin Dependent kinase (CDK) Dbf + Deplendent Kinase (DDK) - combination with of Cdcy and dbf+ Steps- * Cde 45 protein is compound sohich is need of Pre-RC into initiation complex. for the conversion Its binds with wa Chromatin after the begining of initation in late 61 phase by physically associated with Mem5. * binding of Cde 45 is based on Clb-Cde the function of ede 6 and Mem. 28 and as wellas . VWD ДЕСНИГОCA DEHIK * The activity of Cdt 1 during the cell cycle is regulated by a protein called Geminin. * It also inhibits Cde 1 activity during the s phase in order to prevent the re-replication of DNA Ubiquitination and proteolysis.