University:
Coventry University
Course:
115 BMS | Introduction to Biomedical Analysis
Academic year:

2023
Views:

451

Pages:

3
Author:

Badusha S

AIM: To study the effect of temperature on the enzymatic activity of salivary amylase on Substrate starch. PRINCIPLE salivary amylase or Phyrelin is an enzyme. Present în saliva secreted by the salivary glands This enzyme acts cupon starch and converts it into maltose All enzymes work at an optimum km pt and pH If kmpt and pH is not suitable, then Ouivuly of the enzyme, gets affected adversly. Materials required: Test tube test tube stands, beakers, Pipettes, funnels Spirit lamp, thermometer measuring cylinder cotton, starch, lodene, Potassium 10dide, sodium chloride thermord box. Procedure: collection of saliva: Make a thin sheet of cotton and dip it into tap water brain off the water of the cotton Spread over the funnel. keep the funnel over a clean test tube. Now chew a piece of rubber and Pour the saliva into the funnel. The filtered saliva is collected at the bottom of the best tube. Preparation of starch sons: Pake 100ml of coater in a beaker and boil it. Mean chile add 19 starch powder to a little coater and make a pasle. Add this paste to the boiling AIM: To study the effect of temperature on the enzymatic activity of salivary amylase on Substrate starch. PRINCIPLE salivary amylase or Phyrelin is an enzyme. Present în saliva secreted by the salivary glands This enzyme acts cupon starch and converts it into maltose All enzymes work at an optimum km pt and pH If kmpt and pH is not suitable, then Ouivuly of the enzyme, gets affected adversly. Materials required: Test tube stands, beakers, Pipettes, funnels Spirit lamp, thermometer measuring cylinder cotton, starch, iodine, Potassium 10dide, sodium chloride thermord box. Procedure: collection of saliva: Make a thin sheet of cotton and dip it into tap water brain off the water of the cotton Spread over the funnel. keep the funnel over a clean test tube. Now chew a piece of rubber and Pour the saliva into the funnel. The filtered saliva is collected at the bottom of the best tube. Preparation of starch sons: Pake 100ml of coater in a beaker and boil it. Mean cohile add 19 starch powder to a little coater and make a pasle. Add this paste to the boiling. to the action of enzyme salivary amylase i Pake a few drops from test tube B and added 3 drops of iodine soln. A dark blue colour appears It shows the presence of starch in the soln.