University:
North Seattle College
Course:
CHEM 255 | Biochemistry I
Academic year:

2023
Views:

22

Pages:

14
Author:

Christopher Brooks

Glycolysis:- also called glycolytic pathaway Con Embdenmeyerhoff- Greek-glykys = Sweet Lysis = Splitting Parnas (EMP) pathway. -The whole proces of glycolysis occurs in ten steps First five steps are Preparatory phase (1-5) Neat five steps are. pay off phone (5-6) In Preparatory phase there is phosphorylation of glucose and its conversion to glyceraldehyde. -3-phosphate at the expense of two molecules of ATP -in payoff phase then is oxidative conversion of glyceraldehyde-3- phosphate to pyruvate atad the formation of ATP & NADH The Sum f The 2 Sequential ixne would be G-3-P + NAD+ + PR' +APP 3-phosphogly cerale + ATP + NADH +HT Net Outcome of Step 6 & 7 ú: (a) glyceraldehyde- 3-phosphalt, an aldehyde in oxiduad to 3-phosphogly curate a carboxylic acid b) NAD+ is reduced to NADH c) ATP is produced from ADP & Pi The net geult of step 62 7 is that the energy released on oxidati (or dehydrogenation) of an aldehyde to a carbonylate group by the coupled formation of ATP Such reactions phosphorylation is conserved from ADD & Pi are called Substrale Level - Step-8 Isomerization of 8 phosphoglycerate The 3-phosphoglycereale is converted into 2-phosphoglycerale clue to the intramolecular shift of phospho seyl group from Cz to C₂ by the enzyme phosphoglycerali mutate. The i a geversible ex Phosphogly cerale Mutase. It is a dimex of identical subunite. >mot.cot 65,000 12t is essential for this exn Mg2+ is -> The enzyme reeg's 2, 3 diphosphoglyceralt as cofactor Enzyme -OH + 2,3 diphosphogly cerate En zyme- O.Po +2, 3 phrepto glycati > At high conc of 3-phosphoglycerate , the 3-phospho glyceralé & phospho enzyme react to produce free enzyme 2 2,3 duphosphogly cerale yields the 2-phosphoglycerate. which in twin The sixn occus in of 2-phosphogly ceralt. revere dered at hugh Core Af Equilibreewin the ratio of phosphate й 50:1 2 to Hae 8- 3 phosphogly cerati + ATP 2,3 diphosphiglyeuli + ADP. 19. Dehydration of 2-phospho gly cerali > The 2-phospho gly cerats is dehydrated by the action of Enolare to phosphoend pyruvale. > The is a reversible rxn The loss of H20 Cames redistribution of energy withen the mod gausing the phosphate zon position 2 to the high change. stale thus forming PED. H-C - O. PO2 Enolare Mg2+ Mo²+ 99 +H₂O 2 -0.PO CH₂ phospho enol pyruvalo CH₂OH 2-phospho glycerale Enolase - Ils a dimes with identical subunité - mol cot - 88,000 - 12+, Mn 2+ Ion 2+ of Cu2f Regi My 24, as cofach. Step 10 Transfer of phosphato from PEP to ADP This is the second And generating srxar in glycolysi PEP is converted to pyruvate in Enol form by the inducible allonterec Enxyme Pyruvate kindve The Enzyme Catralyxes the transfer of a phosphay) go tham PEP to ADP thes forney ATP