PEPTIDES AND PROTEINS:
- Proteins are polymers made from amino acids and they form peptide
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The process of maxing proteins from amino acich in a condemation reaction that leads to the formation of water morcules.
alanine glycine:
HN-or-coon + HN-CHE-COOH N,N-CH-CO-N11-01₂-COOH + H₂O
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Proteim cue alho broken down to form amino acids, by hydrolysis. We can carry out acid or bare hydrolysis. -
Acid hydrolysis:
HN-CM-CO-NH-01-1001+ HD 12H+ H₂N-CH-COOH + H₂N'-CH-COOL دائی
CH
The mixture of amino acids can then be separated waeg paper chromatograph GI TLC
Amino acids are colourless hence a locating agent must be sprayed on the chromatogram, to help identify the paritions of the amino acido.
Rf. values are inen worked out, to help delamine the aming acido present
* If back hydrolysse in carried out the excess bone reacts with the carboxylic group to porin carboxylate ton).
HN-CH-CO-NH-CH-COOH 1201→ 11N-CH-100N-CH-00
CH
CH