A Congenital Diaphragmatic Hernia is a birth defect that causes a tear in the diaphragm that pushes abdominal organs into the chest cavity, therefore, preventing proper lung development. CDH occurs in one out of 3,000 live births, and has a high mortality and morbidity rate (POBER 2007), with survivors requiring high technology-driven interventional perinatal care (STOLAR 2012) in tertiary or...
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In the left lane 5 bands were obtained by digesting lambda DNA with EcoRI. This depicts that the DNA has been digested as first few lanes are visible instead of 12 visible bands, this is due to weak staining of DNA, hence only larger bands are visible. Also, agarose gel does not have a high resolving power as compared to...
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Abstract This paper will discuss the methods and applications of gel electrophoresis. This researcher will address the production of a gel from agarose and a buffer solution. The role of the gel, the buffer solution, and the intricacies of the electrophoresis machine will be capitalized upon. The laboratory experiences of this researcher in extracting DNA from plant-based materials will be...
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The process of DNA Electrophoresis is very intricate; there are many factors that can push the experiment either way. If done right Electrophoresis can help change the world in a dramatic way. The most advanced discoveries have come from DNA Electrophoresis like: cloning, DNA Fingerprinting, and the discovery of DNA’s shape and form it takes. DNA Electrophoresis is a long...
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DNA is composed of three parts- a phosphate group, a 5-carbon (pentose) sugar called deoxyribose, and a nitrogenous base. In DNA, there are four kinds of nitrogenous bases. These bases are called adenine (A), thymine (T), cytosine (C), and guanine (G). DNA is a negatively charged particle, and its negative charge comes from the phosphate component. “The backbone of DNA...
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Our bodies are a complex system of bones, muscles, vital organs, water, and blood that has been built upon since we were embryos. What created how our bodies were built was on Deoxyribonucleic acid or DNA for short. It is a system of an ever-complex combination of 4 different nitrogen bases that expand to over 3.2 billion combinations that are...
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Imagine a future in which parents can give their unborn child the best genetic traits possible, grooming them for genetic perfection. Through genetic testing and gene therapy, modifying and enhancing genetic attributes is becoming a reality. These industries are growing rapidly, with 1,000 genetic tests already available to the public (Papalia and Martorell 61). Genetic testing and gene therapy have...
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The CRISPR-Cas9 technology has made editing DNA so uncomplicated and inexpensive that amateaur scientists and even average people are beginning to dabble in the subject for things like dog breeding and agriculture. However, genome editing actually has the potential to completely transform the modern medical world with new approaches to the creation of treatments that could change lives. The significance...
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Introduction You have asked me to research DNA test kits, which are tests that analyze a sample of an individual’s DNA and look for specific characteristics associated with different conditions. These tests can reveal genealogical information, health information, and also establish legal paternity. DNA tests can also be used in forensic science to help solve active cases. Background DNA, which...
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The Foundation of CRISPR Clustered regularly interspaced short palindromic repeats or CRISPR as it is more commonly known would not be possible without the many scientific discoveries that enabled scientists to understand DNA. Many discoveries enabled this, such as Watson and Crick and Rosalind Franklin, however the most important was the use of recombinant DNA.The first use of recombinant DNA...
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The field of genetic engineering and specifically gene editing has grown significantly in the last decade with the creation of Clustered Regularly Interspaced Short Palindromic Repeats - Cas9, or CRISPR, and the emergence of gene manipulation. This technology can be very risky or valuable based on how it is used. Using Gene therapy, all genetic disorders in humans and animals...
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Introduction Shelf life of food is the maximum time that the food produce can be stored, during which duration the quality remains acceptable for storage. Food industries face major challenges in storage and transportation after harvesting of fruits due their highly perishable nature. Ripening is followed by decaying due to accumulation of toxic components which Is the result of increased...
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The use of DNA evidence in courts have grown in recent years, in fact, DNA testing has over the years helped law enforcement identify as well as solve difficult crimes. DNA evidence has over the years has helped prove that most convicted people are, in fact, innocent. However, although DNA evidence can be accurate, there is often a danger of...
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Introduction Eugenics is the philosophy and social movement that argues in favor of human advancement and engineering. Eugenics can be dated all the way back to 1883 with Sir Francis Galton who proposed being “well-born”, which is the idea of selective procreation with “desirable traits” (Genetics Generation, 2015). Eugenics is a highly debated topic and is somewhat frowned upon because...
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DNA isolation is a process that deals with the purification of DNA from a given sample with the help of physical and chemical methods. According to -, DNA was first isolated by Friedrich Miescher in 1869. Of recent, it is a routine procedure used in both molecular biology and forensic analyses. In regards to the chemical method, there are many...
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Introduction Protein–DNA interactions play a critical role in the molecular biology of all organisms. For example, the ∼3.3 billion base pairs human genome is estimated to code for at least several thousand DNA-binding proteins, including transcription factors, nucleases, repair proteins, topoisomerases, structural proteins, and DNA and RNA polymerases. The experimental research aims to isolate DNA plasmid from an E. coli...
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There are many different types of viral vectors that are used to deliver a corrected CFTR gene. The Adenoviral vector was one of the first vectors to be tested to see if it couls successfully deliver the corrected gene. Ad vectors don’t become a part of the host genome and remain episomal which means they can replicate independently. Just a...
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The DNA Structure is important for determining the structure of protein.The DNA is located in the nucleus.The nucleus is an organelle found in cells. Inside its fully enclosed nuclear membrane, it contains the majority of the cell's genetic material. The purpose of DNA is that it controls and gives cells to our entire bodies.Such as: genes, lungs, fingers, skin, etc....
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Abstract In this paper, we introduce genomics, study of the structure, function, evolution, mapping, and editing of genomes. Genomics determines the sequence of molecules that make up DNA of an organism. Here, the genomes play a vital role. Genomes can be encoded as well as decoded. So, here we see how to decode a genome with the machine learning concept....
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CRISPR (clustered regularly interspaced short palindromic repeats), which is a technology allowing scientists to edit genes in living cells, has ignited a revolution. Even though it is a relatively recent discovery in the history of biomedicine, CRISPR has entered mainstream use in research swiftly. In just less than a decade, the technology has taken off like a skyrocket with tremendous...
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Long before gene-editing technology became available, public debate over the ethics of using the technology to treat humans had been raged. Immense promises and worst fears associated with modern biomedicine reasoned the intense ethical interest in the technology. All believed that there was something exceptional, something groundbreaking about this new science and technology. Gradually, the debate moved from general philosophical...
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Introduction The question of how to define what a species is has been a contentious issue for as long as the concept has been around. Even without a consensus on definitions the idea of a species is fundamental to all fields of biology, especially so with evolutionary biology. The term “I know it when I see it” can be a...
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INTRODUCTION Motor neuron diseases (MNDs) are a group of neurodegenerative diseases with progressive degeneration of motor neurons (MNs) that lead to muscle weaknesses, loss of ambulation, and chronic disability, ultimately causing premature death in patients (Cappella et al., 2019). Two representative examples are spinal muscular atrophy (SMA) and amyotrophic lateral sclerosis (ALS), both severe MNDs that share pathological, cellular, and...
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ABSTRACT To take complete advantage of the large and rapidly increasing body of sequence information, advanced technologies are very much required. Among the most powerful tools for genomics are high-density arrays of oligonucleotides or cDNAs. Exploration of the genome using DNA microarray should narrow the gap in our knowledge between gene function and molecular biology. Nucleic acid arrays or simply...
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For those that watch television crime dramas, the use of DNA evidence appears to be the final nail in the conviction coffin. Television portrays DNA as direct evidence; evidence that proves the existence of the fact without the use of interferences or presumptions. In reality, DNA is wholly circumstantial in nature. In this paper I will be focusing on what...
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INTRODUCTION For this topic, I plan to discuss eugenics and the forced sterilization of women in the early 20th century. I plan to explain how mostly the disabled, immigrants, and minorities were targeted as well as the implications of that. I will explain the racist and ableist roots of this era of American history, and how it’s tied with the...
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Diseases come in various types and subtypes and they all possess diverse underlying mechanisms. A common factor in all of them is the influence of the genetic background that is unique to every individual. The presence of susceptibility genes and modifier genes in our genome provides insights into the way our bodies respond to disease-causing factors and the complicated gene-gene...
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Involuntary sterilization in the United states started with the Eugenics movement in early 20th century. Eugenics was a compulsory sterilization movement that was of decreasing non-favorable population, including disables, feeble-minded and non-Anglo people in US. (Barnett, 2004). Although the idea of manipulating human reproduction to improve the species has been traced back to Plato's Republic (circa 368 BCE) and beyond,...
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Abstract Horizontal gene transfer (HGT) has a great role as a driving force in the evolution of prokaryotes and eukaryotes and has occupied a major role in evolutionary thinking. Many studies have shown how prokaryotic evolution has been manipulated by HGT such as the spread of antibiotic resistance in bacteria. The importance of HGT in eukaryotes has long been eclipsed...
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Abstract In today’s society, there are numerous children that are born with a childhood disease that is life-threatening. Many children do not live past the age of five with some of these debilitating diseases. As a result, researchers are tackling the crisis by editing the mutated gene of the child while in gestation. Therefore, this will eliminate the possibly that...
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