University:
Boston University
Course:
CAS BI 108 | Biology 2
Academic year:

2024
Views:

468

Pages:

1
Author:

Natalie Patton

CELL SORTING DATA ACQUISITION! FACSDiva! - gating! - % populations! - number of ! cells sorted! - compensation! WHAT TYPE OF FILES SHOULD I ASK FOR?! Raw FCS files! ! particle fluorescence and light scatter! NO GATING DATA! NO SORT DATA! ! good for fluorescence analysis! ! ! FlowJo ! Data analysis station! or lab computer! ! - analyzes cell fluorescence data! ! ! ! FACSDiva experiment! ! contains raw FCS files PLUS:! gating data! sort data! ! the most full record of your experiment BUT can be opened in FACSDiva only ! ! FACSDiva! Data analysis station! ! - analyzes cell fluorescence data! - can produce plots for publication that include sort info (gating etc) ! ! PDF! ! gating info! some stats! NO FLUORESCENCE DATA! ! good for record keeping! ! GRAPHS STRAIGHT FROM THE SORTER ARE NOT PUBLICATION! QUALITY!