University:
Boston University
Course:
CAS BI 525 | Biology of Neurodegenerative Diseases
Academic year:

2024
Views:

387

Pages:

2
Author:

Maximus Barr

General guidelines on number of mice needed for rederevation Type/Strain Age Males Females (min-max) (min-max) (min-max) Excellent Breeders Males: 2-9 mo. (proven, and line produce litters of 6+ pups) Female: 3-10 wk Average Breeders Males: 2-7 mo. (not proven, line produces < 6 pups per litter) Female: 3-10 wk C57Bl6 background Female: 3-8 wk 3-5 4-5 3-5 6-10 3-5 Depends on breeding efficiency of line (excellent or average) 3-5 Depends on breeding efficiency of line (excellent or average) 3 males 6-8 Females (can be purchased; age is strain dependant) Males 2-7 mo. (< 6 preferred) FVB background 5-10 wks (7 weeks preferred) Males:2-9 mo (<6 preferred) Heterozygous Lines 2-10 mo. (<6 months is preferred) Note: Males being used should be set up with females 2 weeks prior to rederivation for 3-5 days, and then housed without female for 5-7 days before rederivation. The following table was outlined by LASC and the Transgenic Core. PROCESS STEP DAY Day 0 Day 1 Day 3 Day 4 Day 5-‐25 Day 25 Day 26-‐46 Day 46 Day 47-‐74 Day 74 Day 75-‐84 Day 84 Mice are transferred to rederivation protocol Prepare PMS Injections Provide PMS Injections Prepare HCG Injections Provide HCG Injections Set up donor breeder pairs Set up recipient females with vasectomized males Make up M2 medium for culture and wash steps Plug Checks on donors females Plug checks on recipient females Oviduct Collection Make transfer needles and anesthetics for rederivation Wash and delivery to Transcore Isolate and wash embryos 5 times before implantation Implantation Surgery & recovery Daily Husbandry Post-‐op monitoring and analgesia Birth of Litter Daily Husbandry Wean litter and transfer to LASC quarantine room Daily Husbandry Collect and send out serology/fecal samples Daily Husbandry Read sample results and release from quarantine GROUP LASC Transcore LASC Transcore LASC LASC Transcore Transcore LASC Transcore LASC Transcore LASC Transcore Transcore LASC Transcore LASC LASC LASC LASC LASC LASC LASC