Estimate the pl Value of the Following Hexapeptide | Boston University - Edubirdie

University:
Boston University
Course:
CAS CH 214 | Organic Chemistry with Qualitative Analysis
Academic year:

2024
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485

Pages:

1
Author:

Lafuentedqri

Es#mate the pI value of the following hexapep#de: Phe-‐Lys-‐Asp-‐Cys-‐Thr-‐Tyr Step 1: Determine the total posi2ve charge on the pep2de when all acidic and basic groups are fully protonated (at low pH). Step 2: Determine the total nega2ve charge on the pep2de when all the groups are 2trated (at high pH). Step 3: List the pKa values of all acidic and basic groups in order from lowest (pKa1) to highest. Step 4: Calculate the pI as the average of the values for pKa value of the proton dissocia2on forming a neutral species from a +1 species, and pKa value of the proton dissocia2on forming a –1 species from the neutral species. this pep2de So for Step 1: charge when fully protonated +2 Step 2: charge when fully de-‐protonated -‐4 Step 3: pKa values are: 9.0(N-‐term), 10.5(Lys), 3.9(Asp), 8.4 (Cys), 10.5(Tyr), 3.5(C-‐term) List from lowest to highest pKa 3.5 3.9 8.4 9.0 10.5 10.5 Charges +2 D +1 D 0 D -‐1 D -‐2 D -‐3 D -‐4 Step 4: The pI is (3.9 + 8.4)/2 = 6.2

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