University:
Boston University
Course:
GMS BC 630 | Ethical & Policy Issues in Health and Medical Services Emergency Management
Academic year:

2020
Views:

419

Pages:

18
Author:

Clint W.

MATERIALS & METHODS Preparation of Magnesium Chloride Magnesium Chloride Magnesium Chloride Steps of Human Dental Pulp Cells (HDPCS) Extraction G H Human Dental Pulp Cells Cultured with Magnesium Chloride Concentrations Freshly Extracted Third Molar Teeth Primary Passage of Human Dental Pulp Cell Culture Secondary Passage of Human Dental Pulp Cell Culture Magnesium Chloride (MgCl2) Control Control 0.5 mM MgCl2 1 mM MgCl2 2 mM MgCl2 4 mM MgCl2 8 mM MgCl2 Outcome measures Attachment Efficiency 4, 8,12,16 and 20 hrs Proliferation 7,10,14 days Differentiation 7,10,14 days ALP DSP DMP1 DSPP Mineralization 7,10,14 days RESULTS RESULTS 120 Cell Attachment Effeciency (%) 100 80 60 40 20 0 Control 0.5 mm 1mm 2mm 4mm 8mm Concentrations of Supplemental Magnesium Chloride (MgCl2) mM Histogram showing Cell Attachment Efficiency at 16 hours of all Magnesium Chloride (MgCl2) Concentrations Control 0.5 mM 1 mM 2 mM 4 mM 8 mM 50 b b 45 b 40 b 35 b Cell Proliferation (Folds). 30 b b b c c 25 20 c c 15 a 10 5 0 a a a a a Day 3 b b a a d Day 7 c d Day 10 d d d Day 14 Histogram showing Cell Proliferation Rate of Magnesium Chloride (MgCl2) at Different Time Intervals d d Day 21 Time (Days) Control 0.5 mM 1 mM 2 mM 4 mM 8 mM 120 a a 100 b b Cell Viability % 80 60 a a b c c a b b b b b b c c d d e 40 f e f e f c d d 20 0 16 hrs Day 3 Day 7 Day 10 Day 14 Time (Days) Histogram showing Cell Viability of Magnesium Chloride (MgCl2) at Different Time Intervals d 1.4 Control 0.5 mM 1 mM 2 mM 4 mM b 8 mM 1.2 b Cell Proliferation (Folds) 1 0.8 b 0.6 b 0.4 b a b a a a c a a d a a e d 0.2 0 Day 7 Day 10 Day 14 P<0.0001 Time (Days) Histogram showing Cell Proliferation Rate of Magnesium Chloride (MgCl2) at Different Time Intervals (Odontogenic Media) Control 3.5 0.5 mM 1 mM 2 mM 4 mM 8 mM b 3 c ALP Activity (mU/ml ) Per Million Cells 2.5 b b c c b a d 2 d 1.5 c d 1 0.5 0 a b a a e f Day 7 Day 10 Day 14 P<0.0001 Time (Days) Histogram showing Alkaline Phosphatase Activity of Magnesium Chloride (MgCl2) at Different Time Intervals Dentin Sialoprotein Expression (ng/ml) Per Million Cells Control 0.5 mM 1 mM 2 mM 4 mM 8 mM 500 b 450 c d 400 350 300 b 250 c d a e e f f 200 150 100 50 0 c a b Day 7 d a a e Day 10 Day 14 Time (Days) Histogram showing Dentin Sialoprotein (DSP) Expression of Magnesium Chloride (MgCl2) at Different Time Intervals Control 600000 0.5 mM 1 mM 2 mM 4 mM 8 mM c b 500000 Dentin Matrix Protein Expression (DMP-1) (pg/ml) Per Million Cells 400000 b c a a b c d 100000 0 Day 7 e e a d f e 300000 200000 d f f Day 10 Day 14 P<0.0001 Time (Days) Histogram showing Dentin matrix protein1(DMP-1) Expression of Magnesium Chloride (MgCl2) at Different Time Intervals Dentin Sialo phospho (DSPP) Protein Expression (ng/ml) Per Million Cells Control 0.5 mM 1 mM 2 mM 4 mM 8mM 500000000 c 450000000 b 400000000 d 350000000 300000000 c 250000000 b 200000000 50000000 0 a b d e a 150000000 100000000 a c d e e Day 7 f f Day 10 Histogram showing Dentin Sialophosphoprotein (DSPP) Expression of Magnesium Chloride (MgCl2) at Different Time Intervals Day 14 P<0.0001 Time (Days) f Minerlization Rate per million cells Control 0.5 mM 1 mM 2 mM 4 mM c 9 8 b 7 6 c 5 4 3 2 8 mM a b c c c d b d d e d e a f a 1 0 Day 7 Day 10 Day 14 Time (Days) Histogram showing Mineralization Rate of Magnesium Chloride (MgCl2) at Different Time Intervals CONCLUSIONS • Conclusions • This is the first report to investigate the optimal magnesium concentration needed to induce human dental pulp cell attachment, proliferation, differentiation and mineralization • Conclusions • This study showed that HDPCs with 0.5mM and 1 mM magnesium supplemental groups elicited the highest stimulatory effect on cell attachment efficiency, cell viability, proliferation rate, ALP activity, expression of odontogenic-related proteins (DSP, DMP-1, DSPP) and mineralization at all time points Conclusions • However, 8mM magnesium group had an inhibitory effect on HDPCS behavior showing lower ALP activity, expression of DSP, DMP-1, DSPP, and mineralization compared to the control