Staphylococcus Aureus

STAPHYLOCCUS AUREUS Noraxella •gram positive except recillonelle Neisseria All cocci ave Localizel pyogenic insectin to syst. inf.immen. CLASSIFICATION MILEO COCCACEA STREPTOCOCCACEA STAPHYLOCOCCUS streptococcus Staphylococcus Enterococcus Micrococcas Feature Tetrad shope Bund of grapes oxidative alucose utilinto Ferreritative Hugh leif wh Mediun Indicate TETB Oxidase Catalase Bacitracin Resistant sensitive. golden color pigment staplylotomi STAPHYLOCOCUS (AUREUS > Bunch of grapes > Non-Motila Non-Poring > Hetalase (H) >xidase (-) VIRULENCE FACTORS Cell wall ass Peptidoglycon, Techoic acid. Cell warfare Protein A Anh-complementing Anti-phagocytic Co-agglutination Gagulase Rom CLUMPING FACTOR 119 T →COWAN-I-STRAIN of sarous ✓ Toxins att k FigG Enzymes TO XINS Homoly sins Alpha -Lyza shrap & Human RBC -Inactivard at 70°C (Activation at 100°c) Beta -Hot cold phenomonst @37Hemolysis -Lyse sheed RBC my @4°C Hemolysis accentuated. Gouma opelta o Panton-Valentine toxin (Methiullia-Resul) Secreted by 2-5/ MRSA (Synerga hymenotrophic toxin PU+ hemofsin • Epidermolytic/Exofoliative toxin 4. Straphybcoccal scolded skin Sy • Ritters disease (children) TEN (Toxic epidermal necrests) •Enterotoxin A1(GF) R-TV (FOOD POISONING) o Food poisoning (Dairy, Meat) Hoat stable OVAGOMIMETIC ACTION emosis Incubation Period = 1-6 hrs TOXK SHOCK SYNDROME TOXIN (Prolonged use of Tompons) enterotoxin + Superantigen (Massive cytokine) Release ENZYMES Catalase. cocqus STAPHYLOCOCCUS PHYL Thermo niclens Phosphate conguluse DNAas Lipase INKAL FEATURES s-Skin, sc Inleaka 0-3 OM. F-Food Poisoning T-TEN, TSS S/O/F/T/P/A/I/N/S P-Preumonia, Pneumatocele A-1 uule endocarditis I- Infective Arthritis 19 N-Necrotising fascitis s- Sepsis DIAGNOSIS • MICROSCOPY Grom + cocci CULTURE A Blood culture for Bsli LAB DIAGNOSIS Anh Liotic sensitivit teshj Typing of S. aureus -Nutrial uger S. Aureus golden pigments Staphylloxanthia BLODA AGAR B-Hemolysis -narrow zale of -+ Selective media 7-10% sall resist Mornitol salta gor. Ludlomi ager (2nd Rest) Indicator (R-carotene) Non-diffusible Prendamas diffesle Blue differential media. Salt-milk ager. BIOCHEMICAL IDENTIFICATION ratalase H202 the+Bubbles. CATALASE TEST tsomple. chumping factor ✓ COAGULASE TEST SLIDE COAGULA SE Blood coagulase Staph. Aureus FEATURE AKA Staph. Intmedus staph Hyicus eta LUGDUNENSIS Phenol Red Candida Ecot CATS GENCE BEING MADE CRF readed TUBE COAGULASE Free coagulase Positive in Staph. Schleiferi Identificetin & AUTOMATED SYSTEMS! UITEK, MALDI-TOF NIRSA (Smki) Methicilin resistat :S aurens. ✓Mediated:-Chromosome encoded mec A gene Alpration of Penicillin BP (PBP2).... PBPZa (MRSA mutant PBP) PBPza less affinid for p-lactam Antibiotiotis MRSA resistant + B-lactam Anfibiotics. mec A gene subtype IV, V, VI More virulent (PVL) Types of MRSA Invasive skin & soft tissue infections. Necroting fasciitis Hospital HA-MRSA Mec A gene subtypes 1,1,111 MDR Treatment Vancomycin Causes SSI, HAIS Communit MRSA CA Detection of MRSA Oxacillin screen ager PCR detecting mec A gene - latex agglutinatia test dielecting PBPZa Identificetin & AUTOMATED SYSTEMS! UITEK, MALDI-TOF NIRSA (Smki) Methicilin resistat :S aurens. ✓Mediated:-Chromosome encoded mec A gene Alpration of Penicillin BP (PBP2).... PBPZa (MRSA mutant PBP) PBPza less affinid for p-lactam Antibiotiotis MRSA resistant + B-lactam Anfibiotics. mec A gene subtype IV, V, VI More virulent (PVL) Types of MRSA Invasive skin & soft tissue infections. Necroting fasciitis Hospital HA-MRSA Mec A gene subtypes 1,1,111 MDR Treatment Vancomycin Causes SSI, HAIS Communit MRSA CA Detection of MRSA Oxacillin screen ager PCR detecting mec A gene - latex agglutinatia test dielecting PBPZa