University:
Camosun College
Course:
NURS132 | Promoting Health and Wellbeing
Academic year:

2023
Views:

191

Pages:

3
Author:

Aysha A

PROTOZOANN INTESTINAL AMOEBIASIS Entamoeba histolytica => pathogenic intestinal Anseball cause Amoebic dysentry & AMOEBIK LIVER ABCESS// MORPHOLOGY Precyst Trophozike - Invasive, leeding form, replicating (15-20pm) Psendo podia 1 nudens Cyst (Diafrustic form) 12-15pm (1-4-mud), matare cyst-4 LIFE CYCLE Host:- Man Inf. form Mature quadrinucleated cyst Node of transmission D Feco-cral Route. 2) sexual Vectors (Alies) Excystation lysin (smal intestine) disntegrated Rwtrophozoiles rol. m feces Man aquies Mlection by. 77 ingestion of food co wath contaminated Mative cyst Released in Feces. Cysts are Formed Cimmature & Mature) (Encystatio Trophozoiter are released Carried t Logemlestine Trophozoites cobnice Large intestinal mucose Live abscess Trophowites penetrate intestinal wall und are carried to extra-intestinel sikes// LI, colonize diff conses dep-on host susceptily Asymptomat Cyst passers Cyst Feces Amoebic dyses like intiulcers & dysentry Invasire amoebian's Pork vens Cammen sila Liver VIRULENCE FACTORS Trophozoite of E histolytice = invasive form// AMOEBIC LECTIN ANTIGEN: "adhesion" bind to glycoproten receptors on LI epitheliun • Other virulence, Amoebapore pores un cell, leakage of ions Cysteine proteases & hyorufticeniones. - Neuraminadase & Metallocollagenese - Invasing PATHOGENESIS Colonization (Mlestial mucosa Loz tension Adhesion peretrate mucosa & adhere to LI mucas 5 GalGaNAc lectin molecules. ✓ Flash-shaped ulcers Trophoroites produces FSUL on cecum. Invasion Extraintestinal sites. I cysteine proter Johoshi as // Complications • Fullminant Amoebic Colitis - Generalised necrotic involvement of large intestine immuncomprised patients. occur common in • Amocbic Append acitos Cutaneous amoebiasis Virulent forms per skin ulcers extension of camsabic colitis. Amoeba granulome (Pulpable Ab. Mass) C.M Incubatia-1-4 weeks// - Amoebic dysentry! As cells Bloors dioro hoer + Mucus + coličky, Abd. Pair (X10) Fever, weight loss Amabic appendantes (Right Lour AP) -. Fulminal colitis (>20 motia (der, hypotension) LABORATORY DIAGNOSIS Specimen collection Stool microscopy mount, Ps etc- by wet mount, ✓Stool, min 3, (within lodays) within 1-2hr examined presen in formalin. delect cysts (Round, 1-4 nede and hophozoites (Angr like pseudopodia) gold stunderd Active infecting Brmaned stain Trichome stain/Inon-hematoxylin stain STOOL MACROSCOPY Foul smelling, copious in amount, dork Red colour. Mucus + Blood. HISTOLOGY (At least 6 areas sigmoidoscur Sint Biopsies stained with PAS & H&E stairs trophozoiter/ STOOL CULTURE Polyxenic culture (Bachial supplomat) Axenic culture X STOOL ANTIGEN DETECTON ↳ ELISA dekches 170-KPM of latin Ag Immunochromatographic test (ICT) 29 kDa surface Ay SEROLOGY Amaebi Antigen ELISA (Recent & Active Infection) Amoebic Antibody det IgG ag Amly ELISA (laks stages) Isoenzyme - Electrophoresis > sev zymodeme patterns // MOLECULAR DIAGNOSIS 90% sensit Speutis go-100/- Nested multiplex PCR targeting subunit FRNA genes/ Real time PCR target 185 TRNA +Biofire Film Array