Lecture Note: Corynebacterium

CORNYNE BACTERIOM Cornybacteriven diphtheria morphology:- - gram tul bacilli "Pleomorphic non motile non-sporing Melchic+ facultative ancaseches noo calculated chinese lellel puellen (uniform) arcangemand. • shows clubting gl one of mala buth ends with metachecmodic granules -mado os of polymertophersphalte Bluish purple clove with Los fleis methylene heee. 4 Sp stains for granules demonstratios! -Melet's Heisters Ponders. -155 Pathogens saloons injection:- omnonsite) fauciall Laryngealittacha ENLOVE fenilal Cctaneas beacho Trachoubac nahal Masal otic Conjuclivell prode of transmissive Deplet Virulence factors Wry powerful exotoxin modued by Vi tulske strains diphtheria. -90-95% gravis, inter medies seconds +80-85% -85% toxigenc mitis clean all classmate JASSMA Pork allians 8 strang mainly for toxic fluids. •Toxigenicity:- tox gone a peters Which leads to ull death due to inhibition of theatrin syntheir Pathognicky in RS. Virdend •Usually begins in RS. diphtheria bacilli theoat of sus aptise tonge lodge individual a in •noulliply in spertarial layer of mucus moroke ane te loflammation responce eventually result in preudo membeare. (fiheinous exudate ath during ting epithelial all, leviucifer, erythrocytes thaderie) Wads to mechanical obstruction. -Regianal lymph node of neck often. en layed (ball neck) - Elaborate toxin which causes. nedosis of neighboring tisice alls 'st al Complication of diphtheria!- Local:- Pseudomemheane may extend Tarynx leading to asphyxiate death. Syslome!- Chen to forms myocarditis lead to heart foluere death •Post diphtheritic paralysis of Palatine + ciliary moste Degenerative charges is adrends kidney & liver may ocecul clinical manifestation:- 3-1 days Incobution period :- (may kell day 100)- be Symptoms! Some throat -Sullen lymph. mph node fever chills Moral discharge weakness Lab diagnoss:- Specimen theout swab Swab from Lesions microscopy Cuam stain geam the bacilli with chinese Letter pattern -Albut stan for metachromatic geamuler Coltar Isolation of Bacilli legales media enriched with blood, thum/ egg -Loffles seem slop: Rapid growth, 6-8h. - Pottossum tellurite blood agay wed to tell con Hellarile is ledumed to gives geory / black colour to the colonies Blood afour to pharyngitis, which may stimulate diphtheria. 87 - Aerobic facoltative anerokor. Opt temp-37°C + PH-7-2 3) Vikelonce tests:-(test (or toxicogen. Alovivoters:- animal inoculation (guinea pigs) a-Subcutaneoustest. b. Intracelaneous test B. lo vikotests:- a. Eleks gel doot p aption test b. Tissue cultured lost (Inuinotest -1 (a) Subcutaneustestir one crops cultale of Chewath bus oween offers serumclye he emulsified Sh Binjected to 2 quince pig goined The vildent studio:- Death witho 2-2 with evidence of haemorrhage in adrenal gland. goath from 155 emulsified Inpeeed cutaneously on 2 guim-pl quin Viedent strain- Inflammatory ix's thesile of upt neciyir in 18-724 animal does not dal In ville tests!- a. Tissue celtace tert b. Elekis gel precipital un test! filter papel so saduccalid with antitoxin (1000 untk/ml) is placed on ague plate with 20% horse eeeem. - barleial culture streaked I to filter paper. - tuet we couled put up... - Incubated 9370 for 24-48h. toxicshowo- produces appti line" where it meets anditoxin opt canc Tin toxic stain does not " Teeedmed: Spteeadment must not be delayed if clinical pistare suggests diphtheria. -Ropid suppression forin producing bact with antimicrobial day -early admi & antitoxkssmate 20k- llac unto for celious comes, half the don being to dou bump queen Page Prophylaxis- a) Active immunization (Vacchdon) DPT (Dipthel a toxoid ambi with tetanus toxiu.d petusis) +3 doces are given given g as interv of 4-6 weeks + boortep the age of 18 months + 5 years b. Pastice immunizat 001- ADS (Antidiphtheritic serum) 500-1000unts Subcutanearly c) combined immeni satun:- Ist doxe of DPT+ ADS to he contneed with the full coмсе active immunization