University:
Coventry University
Course:
115 BMS | Introduction to Biomedical Analysis
Academic year:

2023
Views:

229

Pages:

5
Author:

Badusha S

URINE PEST DEST FOR UREA IN URINE: AIM: To test the presence of crea in the given sample of urine. Materials required: urine sample, Phenolphthalin Sodium hypobromite, uriose tablet, glacial acetic acid, methanol, xanthgred solution test tubes et... PROCEDURE 3/ NO: EXPERIMENT Urine Peet:- Pake 5ml of urine în a test tube Add & few chops of phenolphthalin to it so heat the sole became slightly milky Now add a few crystals of crease engyme to the test tube. Sodium hypobromite test: 2. Pake 2ml of the given sample of crine in a test tube and add 5 drops of sodium hypobromite reagent to it OBSERVATION INTERENCE The solution tuins sed Presence of Presence of Urea. 3 xanthous drol test:- Pake 2ml of urine Sample and add 7ml. of glacial acetic and and 2 ml of 10%. xanthydrol solution în methanol Brisk effervescences of nitrogen Presence of urea on standing. Crystals of dimethyl-3 Lirea are formed. Materials required: urine. sample test tubes, con: nitric acid, con: ammonium hydroxide solution, sodium hydroxide solution, Cuso4 solution, million's reagent, sulpho salicylic acid. Albumin Procedure: SI NO EXPERIMENT xanthoproteic Pesa: Pake 2ml of the urine in a test tube Add I ml of con: nitric acid to it. 2. Head the above soln. 3. The above solo is allowed to coul and add ton: ammonium hydroxide solution to it. SULPHO SALICYLIC ACID PEST 2. 1 Take 2ml of crine in a test tube Add 3 crops of 20% sulpho salicylic acid Helle's nitric acid ging Test! 3. Pake sml of con nitric acid in a test tube. Po it add a few urine crops along the test tube's sides. 4 Biuret Pest: Pake fn I ml of the crine a test e! Add tube! Iml of 20% NaOH and a few OBSERVATION white ppt Presence of prokin. Presence of Proteins Presence of Protein. Appeals of yellow colour. yellow colour changes to orange hlhite cloudy PPt. Presence of proteins. Presence of Protein. A white ring is formed at the point of contact of the two solutions violet colour INFERENCE Presence of Proteins drops of cusor solution to it. Million's Dest:- Pake 2ml of urine in a test tube. Add 3 drops of million's reagent to it. mix well and Boil. White PPt gradually turns red upon beating Presence of Protein.