University:
Coventry University
Course:
115 BMS | Introduction to Biomedical Analysis
Academic year:

2023
Views:

286

Pages:

4
Author:

Badusha S

PEST FOR REDUCING SUGAR (Glucose);- i Pake a few drops from test tube A and add a few drops of Benedect's reagent solo to it. Boil the content of the test tube. The yellow ppt shows the presence of glurose. Pake a few chops from test tube B and add A few drops of Benedict's reagent soln. to it. Boil the content of the test tube. No colour change is observed. This shows the absence of glucose. OBSERVATION: Inference: Pest tube A short change of starch solution. inh seducing sugar (glucose) while. B does not show this change. This clearly indicates that heating of the enzyme salivary amylase (pt yalne) donates red of the enzyme. PEST FOR REDUCING SUGAR OBSERVATION TABLE:- Reagent added Lodine solution Benedict's Solution Pest tubes A Room Permperature -Ve (No colour change) +Ve (Red Yellow PPt B contents boiled +Ve (Dark blue-black colour) -Ve (No colour change) PEST FOR SUGAR IN URINE: ALM: To test the presence of sugar în the given sample of crine... Materials required: Benedict's reagent, sample of urine, Fehung's Soln A and B, test tubes. PROCEDURE? SI NO: 1. EXPERIMENT Benelect's Pest: Pake 12ml of urine sample ind test tube Add 2ml of benedict's reagent of OBSERVATION Red Yellow INFERENCE Presence of Sugar it and boil 2. Fehung's test: Pake Q a little urine in a test tube. Add about 2ml solution each of Fehung's solutions A and B In it add and boil. Brown red Ppt Presence of Sugar