Recombinant DNA Technology Introduction - Edubirdie

University:
Coventry University
Course:
B940 | Biomedical Science
Academic year:

2023
Views:

93

Pages:

2
Author:

Badusha S.

Recombinant ONA TechnologyCarrier molecule : Vactor ap of nucleolicle)I lineas or circularIncopacited into hostcell.Target Gene: Target gene is eluted using R.R.Endo nuclease :EnzymesLigase Enzyre:Restriction Endonucleases Nont used in RDN technologyType 1Most specificType 2clause 20 - 30 bp away from / rechiction siteType 34.Typed: 9;EcoR1, BAHI, HIMRestrictionEnzyme: Palundromic in natureGAATTLECORDLETTNA/GTwo type of CetingSticky endL.Bland enduse T4 DNA ligase.use adapters make sticing MC 3 (Multiple ltrung sit. They carry different restrictionP4 Cpoly Lincha's)sitethey are chemicaly or synthassedsiteRestriction endoncebose site size about:4- b7Base of E.N are oral laping.Liguse Enzyme:x VectorTwo type of vector:Cloning VectorExpression Vector

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