Biological Safety Cabinet

BSC Biological Safety Cabinet (BSC) or also called Laminar Air Flow (LAF) is a tool that is useful for working aseptically because the BSC has a regulation pattern and air flow filter so that it becomes sterile and applies UV light several hours before use. with usage procedures asfollowing. 1. Turn on the UV lamp for 2 hours, then turn it off immediately before starting work. 2. Make sure the cover glass is locked and in the lowest position. 3. The fluorescent lamp and blower are turned on and left for 5 minutes. 4. Hands and arms are washed with gemisidal soap/70% alcohol. 5. The interior surface of the BSC is wiped with 70% alcohol or a suitable disinfectant and allowed to evaporate. 6. Put the tools and materials to be worked on, don't overload them because this increases the risk of contamination. 7. The tools and materials that have been inserted into the BSC are arranged in such a way that they are effective in working and create a truly sterile area.Do not use a Bunsen burner with alcohol fuel howeveruse a gas-powered one. Work aseptically and do not let the air flow pattern be disturbed by work activities. 8. After finishing work, leave it for 2-3 minutes so that contaminants do not come out of the BSC. 9. The interior surface of the BSC is wiped with 70% alcohol and allowed to evaporate, then hands are washed with disinfectant. 10. Fluorescent lights and blowers are turned off. WORKING PRINCIPLES OF BIOLOGICAL SAFERTY CABINET (BSC) BSC is a sterilized work cabinet for microbiology work. The BSC has an air flow regulator that creates a dirty air flow (possibly contaminants) to be filtered and recirculated through the filter. BSC is also called a biosafety hood, and is also known as a Laminar flow hood or Class II vertical flow cabinet which provides filtration and air flow that circulates in the work space. The air flow is regulated to prevent outside air from entering and inside air from leaving, to prevent contamination from outside and bacterial contamination from the BSC room. The air that comes out is filtered through a filter so that dangerous cells do not escape into other rooms. The BSC is also equipped with a UV lamp which functions as a killer for microbes in the interior of the BSC.Laminar Air Flow is often referred to as Biological Safety Cabinet(BSC)which is a tool that is useful for working aseptically because the BSC/LAF has a regulation pattern and air flow filter so that it becomes sterile and applies UV light several hours before use. The procedure for using BSC : 1. Turn on the UV lamp for 2 hours, then turn it off immediately before starting work. 2. Make sure the cover glass is locked and in the lowest position. 3. Turn on the fluorescent lights and blower. 4. Leave it for 5 minutes. 5. Wash hands and arms with gemisidal soap / 70% alcohol. 6. Wipe the interior surfaces of the LAF/BSC with 70 % alcohol or a suitable disinfectant and allow to evaporate. 7. Insert the tools and materials to be worked on, don't overfill (overload) because it increases the risk of contaminants. 8. Arrange the tools and materials that have been entered into the LAF/BSC in such a way that they are effective in working and create a truly sterile area. 9. Do not use a Bunsen burner fueled by alcohol but use one fueled by gas. 10. Work aseptically and do not let the air flow pattern be disturbed by work activities. 11. After finishing work, leave it for 2-3 minutes so that contaminants do not come out of the BSC 12. Wipe the interior surface of the LAF/BSC with 70% alcohol and let it evaporate then wash your hands with disinfectant. 13. Turn off fluorescent lights and blowers.