University:
Felician University
Course:
BIO-405 | Genetics
Academic year:

2024
Views:

306

Pages:

13
Author:

Clint W.

The DNA FP 1.EXTRACTION Cells are isolated from tissue Disrupt cells to release the DNA 2. RESTRICTION FRAGMENTS • Restriction Enzymes (REs) are ‘molecular scissors’ that cut DNA @ SPECIFIC base sequences – There are many different types of REs – ex. HindIII looks for the sequence AACGTT and cuts between the 2 As ‐‐ A / ACGTT • When REs cut DNA into pieces, it makes fragments of many different lengths – VNTRs may be in these fragments • You can use > 1 type of RE on a sample 3. AMPLIFICATION • Use PCR to amplify certain pieces of DNA that contain VNTRs or STRs 4. ELECTROPHORESIS ‐ Separates molecules electrically by charge, size, and shape ‐ DNA has a negative charge Size of molecule is next factor SMALLER moves fastest BIGGER moves slower STEPS OF DNA FPING 1. Extraction: DNA is extracted from the cell 2. Restriction Fragments: Cut DNA with restriction enzymes 3. Amplification: use PCR to make copies 4. Electrophoresis: DNA is loaded onto a gel and separated by size 5. Making it Permanent FRAGMENT BAND ... ,,са ...,л Е� о s:::: J: са и, ф " с .s:::: о Ф .,. О -'а>.О s:::: с,:, ФО �о Ф_ ( - (J о ·>.а л \ 4.ug - Е,:, ;о m" u а.а D ...и, -а - 8,ug shirt - - -- ..- - - V·