Lipase Digestion Lab

PhysioEx Lab Report Exercise 8: Chemical and Physical Processes of Digestion Activity 4: Assessing Lipase Digestion of Fat Name: Titus Aina Date: 23 March 2021 Session ID: session-2f855b37-ae97-5b3c-0f20-998abe31c1ba Pre-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. 1 Which of the following is/are true of bile? You correctly answered: It works by a physical process. 2 The substrate used in this simulation is You correctly answered: vegetable oil. 3 When fatty acids are liberated by lipase, the pH You correctly answered: decreases. 4 One of the products of the chemical digestion of lipids is You correctly answered: fatty acids. Experiment Results Predict Question 1 Predict Question: Which tube do you think will have the highest lipase activity? Your answer: tube 3 (lipase, deionized water, bile salts, pH 9.0 buffer). Stop & Think Questions 1 What does the pH measure? You correctly answered: both lipase activity and fatty acid release. 2 Why is it difficult to detect whether lipase is active in tube5(lipase, vegetable oil, bile salts, pH 2.0 buffer)? You correctly answered: The pH is already very low, so a decrease in pH might be difficult to detect. Experiment Data Tube No Reagent 1 1 Lipase 2 Lipase Vegetable Deionized Oil Water 3 Lipase Deionized Water 4 Reagent 2 Vegetable Oil Reagent 3 Time (min) Temp (°C) pH 60 37 6.21 pH 7.0 Buffer 60 37 7.00 Bile salts pH 9.0 Buffer 60 37 9.00 Deionized Vegetable Water Oil Bile salts pH 7.0 Buffer 60 37 7.00 Bile salts Reagent 4 pH 7.0 Buffer 5 Lipase Vegetable Oil Bile salts pH 7.0 Buffer 60 37 7.00 6 Lipase Vegetable Oil Bile salts pH 9.0 Buffer 60 37 9.00 Post-lab Quiz Results You scored 75% by answering 3 out of 4 questions correctly. 1 What is the product of lipase hydrolysis? You correctly answered: fatty acids. 2 From your results, which pH is ideal for pancreatic lipase digestion? You correctly answered: pH 7.0. 3 Which tube confirms that there is no lipase in bile salts or vegetable oil? You correctly answered: tube 4 (deionized water, vegetable oil, bile salts, pH 7.0 buffer). 4 Where in the body does pancreatic lipase hydrolyze triglycerides? Your answer: mouth. Correct answer: small intestine. Review Sheet Results 1 Explain why you can't fully test the lipase activity in tube5(lipase, vegetable oil, bile salts, pH 2.0 buffer). Your answer: The lipase activity in tube 5 cannot be fully tested because the pH is already low in tube 5, and measuring the lipase activity would decrease the pH more. 2 Which tube had the highest lipase activity? How well did the results compare with your prediction? Discuss possible reasons why it may or may not have matched. Your answer: After completing the experiment, it was identified that the tube with the highest lipase activity would be tube 1. This is because it has the closest pH to the intestines. It did not match my previous answer because I did not know the pH of the intestines. 3 Explain why pancreatic lipase would be active in both the mouth and the pancreas. Your answer: Pancreatic lipase would be active in both the mouth and pancreas; it has the highest active activity in areas with a pH of 7.0, like the mouth and pancreas. 4 Describe the process of bile emulsification of lipids and how it improves lipase activity. Your answer: The process of bile emulsification of lipids occurs due to bile physically breaking up the fat globules. It improves lipase activity because it was breaking it down physically and not chemically.