University:
Saint Louis University
Course:
PATH 0100 | Introduction to Pathology
Academic year:

2024
Views:

211

Pages:

3
Author:

brian 11kizz

PRACTICUM VII (URINALYSIS-2) (Glucose) 1. URINE GLUCOSE EXAMINATION (REDUCTION TEST CaraBENEDICT ) Principle - In an alkaline atmosphere, glucose reduces cupric to cupro, then becomes Cu2O which precipitates and turns red. - The intensity of this red color roughly indicates the glucose level in the urine being examined. TOOLS & REAGENTS 1. Test tube 2. Bunsen fire 3. Benedict's reagent with composition - CuSO4 17.3 - Na Citrate 173 - Na Carbonate 100 - Aquadest ad 1,000 ml WORK PROCEDURES 1) Into a test tube, fill successively 5 ml of Benedict's reagent and 8 drops of urine (2.5 ml of Benedict's reagent with 4 drops of urine) 2) Shake, then heat until boiling over a Bunsen fire 3) Or you can put it in a water bath with boiling water for 5 minutes 4) Let it cool, then read the results INTERPRETATION - : Remains cloudy blue or green + 1: Cloudy, slightly yellow green + 2 : Greenish yellow with yellow precipitate + 3: Reddish yellow, with yellow-red precipitate + 4: Orange red to brick red NOTES - Positive reduction does not always mean that the patient suffers from DM, because there are other substances in the urine that are reducing agents. Name any ingredients! - Negative reduction can occur in DM sufferers, because: - Blood glucose levels (kidney threshold value for glucose under normal circumstances 160-180 mg%) - State of kidney function - Mention conditions of glucosuria other than DM! 2. Sediment examination Work procedures: 1. Take approximately 8 ml of urine sample 2. Shake on low speed for 5 minutes 3. Then discard the supernatant (decanther so that approximately 0.5/ml remains) 4. Shake again until homogeneous, take 1 drop and put it on the object glass, cover with a glass cover. 5. Observe under the microscope in a horizontal position, what needs to be observed: -Observe with weak light lower the condenser The diaphragm is slightly closed -Objective 10x Check the entire field of view at a glance, then pay attention if any crystals and pistons are found. Calculate the number per small field of view (IPK) - Objective 45x Pay attention and count the other formations per field of view.The number of pistons reported has an average of small visual field (IPK).The average number of erythrocytes and leukocytes is reported as large visual field (IPB). Sediment elements that are less significant can simply be reported with the following signs: + There is + + Lots + + + Many Name the formations that can be found in acidic urine and alkaline urine, and draw these formations. Lots many