University:
The Pennsylvania State University
Course:
BMB 443W | Laboratory in Protein Purification and Enzymology
Academic year:

2024
Views:

244

Pages:

2
Author:

Alexis H.

Isoelectric point The isoelectric point is the pH at which a molecule or a surface carries no het electrical charge. This implies that the molecule or surface is electrically neutral, as the positive and negative charges present within it balance each other out The isoelectric point is important for several reasons: Protein structure and function side chain alpha-carboxyl alpha-ammonium group group group Understanding the pI helps predict how proteins will behave under different conditions, such as in electrophoresis or during purification processes Solubility The solubility of proteins and other molecules can be greatly influenced by their charge state, which is determined by the pH of the surrounding medium Buffering capacity Buffers can resist changes in pH around their pKa values Drug formulation Understanding the pI of a drug molecule is important for optimizing its delivery and efficacy

Isoelectric Point