University:
Coventry University
Course:
B940 | Biomedical Science
Academic year:

2023
Views:

249

Pages:

3
Author:

Badusha S.

1) Bright feild Microscopy:Bright backgroundStructural sample visualizationlive or dead sample2) Dark Field MicroscopyDark back fieldlooceage will bright.Live & dead sample (Mainly live cell's)No Staining3) Phase Contract MicroscopyLive unstained cellsBlack of white image.Different inthe refractive indendepend on daekel & lighter regionof the sample.4) Differential Inter france Microscopy NoDmaski InterformLive unstained cellAlmos t same as Phase contract image.change in colour is the change in degleeof refractive inder of the Specimen & Sarlandi3D - figure can be constructed. cell Stained with fluencentFluracent dyes : RhodaminTensas red lightLiliolCy3 Orange GgPluressin - GoverningSNARF / svilsensitive to) -ution) HisitYourFluia 2=9<--(sensitive Ca++ 6)Knions6) Immuno Pluracent Midescopyclied to detect specific protein in fined cell.seedDead cells IvilPined cell observedPermiabalizing the cell 19 freetif withlepact Organic solvent or be feegent'saIncubate the cell with primary 'ab' against outprotein of intreast UNT vtalWash it to remove / unbound ab.Add flurencity labelled 2 ob. [20 fe region 1-ab) ofobserve if under pl flu-microscopyAlso known as Indirect Immuno PlusacemMicroscopy 2) Doable label Immuno microscopy.study of mode prts at a bome.3) De convulation Microscopy (type of the )used to create 3-dimensional image.7Avoid outof focus imagemake image moreclear.They focus on 3 plane.Ad a time If Locus on one plane and outof focus next 2.-coonfinue on 3 plane imarge the imgeget clear image.photomodiypoint Scaning C.Mbleathing8) Canfocal Microscopy.Lases Scaning cm1 3 DimageOptimal image9) Two- Photon Exitation Microscopyimage deep in to tissue samples.Reflection15) Total Internal Plusasent Microscopyadvantage help to study the kinatics of cell adhesiskinetic of assumbly &dissombly ofstructures I illuminated.like acfir mices fu bales.Only few ale of coverslip is50 - 100 do nm deapth information can obyget