Spectro Plus Labquest Spectrometer Manual

Use of SpectroVis Plus spectrometer attached to Vernier Labquest To collect absorption spectrum 1. Turn on the Labquest. 2. Using the USB cable, connect the SpectroVis Plus to the Labquest 3. The Labquest should recognize the spectrometer and display on the screen a box that says USB:Abs. Tap in the box and choose “calibrate” off the menu that appears. 4. The screen will change to the calibration screen and begin a 90 second warm-up countdown. While waiting, fill a cuvette ¾ full with deionized water. 5. Insert cuvette into the spectrometer with the clear sides facing the white arrow and sun symbol. 6. When the warm-up is complete, press “finish calibration”. 7. Once “calibration complete” appears on the screen, press OK to return to the main screen. 8. Press “Sensors” on the menu at the top of the screen. 9. Choose “Data collection”. 10. Set the wavelength range to 400-700 nm. 11. Remove the cuvette from the spectrometer. Fill the cuvette ¾ full with the sample. Return the cuvette to the spectrometer. 12. Press
in the bottom left corner of the screen to begin data collection. 13. Wait at least ten seconds for the data collection to take place. Pressto stop collection. Data will be continually collected until you press stop. 14. Additional spectra can be collected into the same file by changing the sample and pressing go again to begin collection. You will be prompted to save or delete the previous data. 15. When you are ready to transfer the data to a computer, unplug the spectrometer and plug in a USB flash drive. 16. Press “File” on the menu at the top of the screen. 17. Select “Export” and navigate to the USB drive. Name the file; it will be exported as .txt which can be opened in a spreadsheet program such as Microsoft Excel. To perform kinetics experiment 1. Turn on the Labquest. 2. Using the USB cable, connect the SpectroVis Plus to the Labquest 3. The Labquest should recognize the spectrometer and display on the screen a box that says USB:Abs. Tap in the box and choose “calibrate” off the menu that appears. 4. The screen will change to the calibration screen and begin a 90 second warm-up countdown. While waiting, fill a cuvette ¾ full with deionized water. 5. Insert cuvette into the spectrometer with the clear sides facing the white arrow and sun symbol. 6. When the warm-up is complete, press “finish calibration”. 7. Once “calibration complete” appears on the screen, press OK to return to the main screen. 8. Press “Sensors” on the menu at the top of the screen. 9. Choose “Data collection”. 10. Change Mode at the top of the screen to “Time-based”. 11. Set the desired rate of sampling and the total length of the experiment. Click “OK”. 12. Back on the main screen, tap in the spectrometer box and choose “Set wavelength”. Enter the value at which to collect data. 13. Press
in the bottom left corner of the screen to begin data collection. 14. Additional spectra can be collected into the same file by changing the sample and pressing go again to begin collection. You will be prompted to save or delete the previous data. 15. When you are ready to transfer the data to a computer, unplug the spectrometer and plug in a USB flash drive. 16. Press “File” on the menu at the top of the screen. 17. Select “Export” and navigate to the USB drive. Name the file; it will be exported as .txt which can be opened in a spreadsheet program such as Microsoft Excel.