# CHOLESTEROL chokes that is On Crystalline scaled. optically active, white A melting point is 149°c It occurs tree animal cellser all animal as fatty exters in first isolated from human galistane The main source of cholestral are fish- liver cils, the brain spinal cond Cattle. of It malecular formula is C₂HD. 41 27 46 structural determination. Determination of Corken skeleton / structura of nucleus. The structural formula of chalestros is u one DH- It forms Confirming the group. acctate when acetylated presence of hydroxyl group. ohe one Adds one double band. molecule of hydrogen [ire, H₂] in presence of catalyst to and also adds bromine to one form chalestral of form dibromide derivative Confirming the presence of double Hydrogenation of chalitoral followed by oxidation with chrisniuon tricerde yield chokertanone, a betone when subjected reduction give a to clemmenson' saturated hydrocarbon, the named cholestone. Thus, we get to know:- me deicble bend. As chiclestanal is loverted to chdastamone Ketones 10, one group is secondary in. nature je; z alcohol C →1-6= C27 454-6 = 2748 This general fermula of tetracyclic compound ie; cholesterol must having 4 rings. diel's hydrocarbo Se at When Cholesteral is disfilled with 360°c, we get diel's hydrocarbon and i LAM OLMA two nucleus getting th inside the then, Ming. hase →eame no of careben atom is retained in dicarboxylic acid. so; keto group not in is present in ring and side chain opening of wing occeres due to - OH grep group Can be prusen't in dicarboxylic Acid methplere group present is flancked on other sides by 'grove hydroxyl gup may beither at be 10; loa zin ring A.. jus Position of OH group at co has been confirmed by: parition of double bond :- The Position of double bond in cholester was establind by following sequence of vreactions. - Formation of trial shows that there occors hyroxylation of double bond [formation of triels shours that cholestral contain one double bond]. jij chalestane trial on oxidation with chromic acid yield a diketone. this indicate there Are two 2º alcohol while the third which is resistant to oxidation; that may be 3 alcohol i oxidation of cholestone diane to a tetracarboxylic acid without loss of an c atom shows that two ketonic must be ring. present on different group bond follows, therefore that the double must be net in in Cholutral Pithient side conly in the rings