University:
Saint Louis University
Course:
PATH 0100 | Introduction to Pathology
Academic year:

2024
Views:

354

Pages:

2
Author:

brian 11kizz

III. URINE GLUCOSE EXAMINATION Examination for the presence of glucose in the urine includes a filter examination. Glucosuria is the presence of glucose in the urine.Glucosuria (excess sugar in the urine) occurs because the kidney threshold value is exceeded.Glucose has reducing properties. Cupric ions are reduced to cupro and precipitate in brick red form. Glucose in urine is determined by a reduction reaction using Benedict's and Fehling's reagents. Another way is to use a dip strip. A. REDUCTION Method : Fehling Principle : In a leachate (alkaline) atmosphere, glucose reduces Cupri (CuO) - Cupro(Cu2O) which settles and is brick red in color. : Fresh urine Material Tools and Reagents Tool : 1. Test tube 2. Bunsen 3. Wood clamp Reagents : - Fehling A (Cupri Sulfate 69.3 grams add distilled water 1000 ml) - Fehling B (K-Na Tartrate 346gr, Na. Hydroxide 100gr, add distilled water 1000 ml) Ways of working 1. Pipette 2 ml of Fehling's Reagent A + 2 ml of Fehling's Reagent B then put it in a test tube (if the Fehling's reagent does not change color then Fehling's reagent can be used). 2. Pipette 1 ml of urine into the Fehling's reagent mixture. 3. Then boil and see the reaction results.Interpretation of Results: - Negative : remains clear blue or green - Positive (+) : cloudy green, slightly yellow - Positive ( + + ) : greenish yellow with yellow precipitate - Positive ( + + + ) : reddish yellow, red yellow precipitate - Positive ( + + + + ) : orange red to brick red Method : Benedict Principle : In a leachate (alkaline) atmosphere, glucose reduces Cupri (CuO) -Cupro (Cu2O) which settles and is brick red in color Material : Fresh urine Tools and Reagents Tool : 1. Test tube 2. Bunsen 3. Wood clamp Reagents : Benedict's Reagent (CuSO45H2O 17.3 gr, Na. Citrate 173 gr, Na. Carbonate 100 gr, add distilled water 1000 ml) Ways of working 1. Pipette 5 ml of Benedict's reagent and then put it in a test tube 2. Pipette 8 drops of urine 3. Then boil and see the reaction results Interpretation of Results: - Negative : remains clear blue or green - Positive (+) : cloudy green, slightly yellow - Positive ( + + ) : greenish yellow with yellow precipitate - Positive ( + + + ) : reddish yellow, red yellow precipitat - Positive ( + + + + ) : orange red to brick red