Morphologies Study Guides

‭Morphologies‬ ‭ ‬ ➔ ‭Exogenous inclusions are mainly malaria and inside the red blood cell after staining with‬ ‭MGG, a bluish ring with Jewish punctuation is highlighted and this is seen in the context of a‬ ‭plasmodium falciparum infection which is one of the main malaria agents.‬ ‭➔‬ ‭Endogenous inclusions reflect disorders in the synthesis of the erythroblastic lineage:‬ ‭ the first is that of reticulocytes which is physiological but we can also highlight red blood cells‬ ✪ ‭which are punctuated, highlighting brown colored grains buff after staining with MGG and this‬ ‭reflects a disorder of heme synthesis.‬ ‭ we can highlight the Jolly bodies and the Cabot rings. Jolly's bodies are bright red dots at MGG‬ ✪ ‭and they are made of deoxyribonucleic acid. Cabots rings are fine filaments arranged in a figure 8‬ ‭in the center of the red blood cell, they are colored red and they represent remnants of the nuclear‬ ‭membrane. These two inclusions represent disorders of erythroblastic maturation.‬ ‭ siderotic (iron) granulations that can be seen physiologically logical in 3/5% of red blood cells, it‬ ✪ ‭is non-heme reserve iron not included in the constitution of heme and we generally highlight it‬ ‭using Perls coloring so we highlight blue colored grains. If there is an increase in the quantity of‬ ‭iron in the blood cells red, there is disruption of hemoglobin synthesis.‬ ‭ Heinz bodies which are not highlighted by MGG but by vital dye: methylene blue and these‬ ✪ ‭Heinz bodies represent the precipitation of unstable/pathological hemoglobins and we will see these‬ ‭precipitations below the cytoplasmic membrane.‬ ‭-Leukocytes:‬ ‭ hese are white blood cells. The leader is the polynuclear neutrophil. There are 5 types of‬ T ‭leukocytes:‬ 1‭ -‬ ‭The polynuclear neutrophil‬ ‭Morphology After‬‭staining with MGG: Small cell, 12‬‭micrometers in diameter. Its nucleus has‬ ‭condensed chromatin in a clump, a nucleus generally segmented into 2 to 5 lobes, lobes linked‬ ‭together by chromatin bridges. We can see hypersegmentation of the nucleus in the context of‬ ‭infectious processes where the organism needs respond very quickly and therefore will synthesize‬ ‭new polynuclear neutrophils and this moment the maturation stage with the segm entation of the‬ ‭nucleus will not be carried out therefore elements will be put into circulation whose maturation is‬ ‭not completely finished but they are functional elements in the majority.‬ ‭ he cytoplasm is pinkish beige in color. We highlight‬‭2‬‭types of granulations:‬ T ‭➔‬ ‭Very fine, bright red granulations which are so-called azurophilic granulations.‬ ‭➔‬ ‭Specific granulations of the polynuclear neutrophil lineage, these are granulations which‬ ‭appear beige brown or buff brown.‬ ‭In fact, there are 5 types of biochemically identifiable granulations:‬ ‭➔‬ ‭red azurophilic granulations which contain peroxidases (such as myeloperoxidase), these are‬ ‭primary granulations and we identify 2 subtypes: those which contain or not defensin which is a‬ ‭molecule which has antibacterial activity.‬ ‭ ‬ ➔ ‭peroxidase negative granulations: There are 2 subtypes: lineage specific granulations which‬ ‭are buff brown in color and contain lactoferrin, lysozyme; the granulations which contain gelatinase‬ ‭which is important for the migration of neutrophils because it will allow the digestion of the basal‬ ‭on which the endothelial cells rest so that the polynuclear leaves the circulatory torrent to go to the‬ ‭tissue level.‬ ‭ ‬ ➔ ‭The 5th granulation is represented by a so-called secretory vesicle which contains a CD‬ ‭differentiation cluster.‬ ‭For these 5 subtypes, the exocytosis speeds of these granulations are different.‬