University:
Texas A&M University
Course:
BIOL 213 | Molecular Cell Biology
Academic year:

2024
Views:

380

Pages:

27
Author:

marthamanayay9qr12

FUNDAMENTAL TISSUES AND MITOSIS Part II Images of light and electron microscopy Objectives • Operate the “virtual microscope” • Describe steps for tissue processing and discuss different types of microscopy • Interpret cytological significance of differential staining by hematoxylin and eosin (H&E) Part II • Recognize and identify cells in microscopic tissue preparations • Understand that morphology reflects the function of cells • Identify stages of mitosis and cellular structures associated with mitosis Laboratory Experience • Primary purpose of this lab is to gain a general impression of similarities and differences in cells and tissues and what can be inferred from that • Do not be too concerned about detailed structure, but rather try to gain an overall appreciation for histology Skin Epithelial skin cells Collagen fibers Skin Tongue (cross section) Skeletal muscle Serous glands Adipocytes Tongue (cross section) Serous glands Adipocytes Skeletal muscle Kidney • Proximal convoluted tubules with microvilli lining lumen Thyroid Spherical epithelial follicles containing thyroglobulin Simple cuboidal epithelium Extracellular collagen fibrils Blood vessel with red blood cells Simple squamous epithelium lining blood vessel Pancreas Zymogen granules Pancreatic acinus Glandular cell with euchromatic nucleus and basophilic base Pancreas Zymogen granules Pancreatic acinus Glandular cell with euchromatic nucleus and basophilic base Endoplasmic Reticulum Granular ER Granular ER Granular ER Nuclear pore Nucleus Nuclear envelope Nucleolus Nuclear envelope Nuclear pore Granular vs. Agranular Endoplasmic Reticulum Granular endoplasmic reticulum Agranular endoplasmic reticulum Plasma Membrane What is the structure of the plasma membrane? Plasma Membrane What is the structure of the plasma membrane? Plasma Membrane Plasma Membrane Identify pinocytotic vesicles Mitochondria Identify mitochondria Mitochondria What is the structure and function of mitochondria? Mitochondria & Microbodies • Could mitochondria and microbodies be confused morphologically? • What is the structure and function of microbodies? Golgi complex Observe the ultrastructure of the Golgi complex Digestion and Storage Vesicles Lipid in SER Secretory granules Lysosomes Digestion and Storage Vesicles Lipofuscin granules Glycogen Microbodies Glycogen Cytoskeleton Microtubules Axonemes encased in membrane Centrioles Cytoskeleton • The microvilli in this micrograph contain what type of cytoskeletal element? • Hint: What is supporting the microvilli? Lipid in SER Cytoskeleton • The microvilli in this micrograph contain what type of cytoskeletal element? • Hint: What is supporting the microvilli? Slide 33 Lipid in SER Clinical Correlation • Cell death can occur due to apoptosis or necrosis • Apoptosis • Programmed cell death • Necrosis • Accidental cell death resulting from pathological condition Max Planck Institute for Neurological Research