BASIC MEDIA CREATION Tool 1. Spray bottle 2. Petridish 3. Erlenmeyer 250 ml and 500 ml 4. Micro pipette 5. 250 ml, 500 ml and 1000 ml beakers 6. Milli pipettes 10 ml and 25 ml 7. Analytical scales 8. Hot plates Material 1. Cotton 2. Tissue roll 3. Spiritus 4. Alcohol 5. Aluminum foil 6. Label paper 7. Aquades 8. Order 9. Meat extract 10. Peptone 11. Dextrose 12. Potato extract PRACTICAL IMPLEMENTATION Making Nutrient Agar (NA) 1. Weigh the medium components with use analytical balances to the desired volume corresponds to the following composition: a. Meat extract 3 g b. Peptone 5 g c. Agar 15 g d. Aquades 1000 ml 2. Divide 1000 ml of distilled water in half, 400 ml to dissolve Meat extract and peptone and 600 ml to dissolve the agar. 3. Dissolve the agar by stirring thoroughly constantly on the hot plate stirrer 4. Dissolve peptone and meat extract just stir it. 5. After both have dissolved, the solution is poured into the agar solution and stir until homogeneous. 6. Measure the pH of the media. If the pH is not neutral then HCl/NaOH can be added. 7. Insert the media into the flask Erlenmeyer and sterilized with autoclave. 8. Pour the sterile medium into a sterile petri dish aseptically. Making Potato Dextrose Agar (PDA) 1. Weigh the media components accordingly with the following composition: a. Potatoes/potatoes 3 g b. Dextrose 5 g c. Agar 15 g d. Aquades up to 1000 ml 2. Boil the potatoes in portions distilled water for 1-3 hours until soft And blackmail him then the extract is taken with filter use filter paper then collected in a goblet. 3. Dissolve the order with a Hot Plate Stirrer in 500 ml distilled water after soluble can be added dextrose and homogenized again. 4. Adjust the pH of the media to 5-6 drip HCl/NaOH 5. Poured media into the Erlenmeyer or into a test tube then it's ready to be sterilized.