Effect of Sublethal Heating and Healing on B. Materials and Tools Culture:  Staphylococcus aureus aged 24 hours (37o C) in TSB medium. Medium/Solution:       90 90 90 60 60 45 mL 0.1 M phosphate buffer solution, pH 7.2 mL TSB mL TSBS (TSB + 7% NaCl) TSA cup agar TSAS agar cups (TSA + 7% NaCl) tubes of 9 mL diluent solution Tool :    10 mL and 1 mL pipettes, petri dishes, test tubes Curved glass rod (hockey stick) Water bath 37o C and 55o C C. How it works A total of 10 mL of culture was put into 90 mL of phosphate buffer solution has been preheated to a temperature of 55o C, then heated inside water bath at 55o C for 10 minutes. With this warming it is expected some S.aureus cells will experience sublethal damage, while some remain normal. Then as much as 10 mL of the culture that has been heated is added to each each into two types of media, namely: 1. 90 mL TSB as a normal medium for cell healing 2. 90 mL TSBS as a stress medium because it contains inhibitory substances healing is salt After shaking well to obtain a homogeneous suspension, second The tube containing the medium and culture is placed in a water bath with temperature 37o C. With healing time intervals of 0 (without incubation), 30, 60, 90 and 120 minutes, 1 mL of culture from TSB and TSBS each was taken and diluted as much as 10-1 to 10-5 . Then 0.1 mL of dilution 10-3 10-4 and, 10-5 each were plated on TSAS agar plates (to count the cells thatnormal). Culture over the agar plate is leveled using a curved glass rod which has been dipped in alcohol, ignited and cooled. All plates were incubated in an inverted position at 37oC for 48 hours. Because of the data collected is class data, then a division of work is made per group D. Observation Results All data obtained were calculated, tabulated and a curve was created shows the relationship between healing time and log number of cells in four various combinations of healing medium and fertilizing medium