Antimicrobial Activity B. Materials and Tools Ingredients:          Media Nutrient Agar Phenol Alcohol Clove oil Sodium hypochlorite Diluent solution Culture of pathogenic bacteria Cultivating food-spoiling yeast cultures Cultivate food spoilage mold cultures Tool:       Petri dish Spirit lamp Round disc Vernier calipers Tweezers Tip C. Work procedures Paper Disc Method 1. Take 1 mL of pure bacterial culture and dilute it in a 101 diluent tube 2. Then shake the diluent tube 8-10 times 3. Take 1 mL, inoculate into a petri dish, add Nutrient agar, wait until congested 4. Meanwhile, prepare antimicrobial solutions in the small glass beaker provided concentration is known 5. Take a paper disc, dip it in the antimicrobial solution until it is all wet 6. Stick it in the hardened agar medium 7. Incubate for 24-48 hours at 37o C 8. Observe the results Agar Well Diffusion Method 1. Take 1 mL of bacterial culture, put it in a petri dish 2. Add NA then let sit until it hardens 3. Sterilely pierce the NA into several sections with a size 6 hole punch mm 4. Then using a micropipette take 100 microliters of antimicrobial andput it in the hole 5. Incubate in an incubator for 24-48 hours at 37o C without turning petri dish