9/25/2020 Results Chem m3 Lab 8 Date / Time September 25, 2020 2:12 pm Chem # Lab 8 Question Student Answer There are three main steps in PCRdenaturation, primer anneling and polymerzation which includes extension and final elongation. Denature step are heated above 90 degree celcius to seperate the two strands. Elongation temperature is increased to 72 c where Taq is most active. This is where the temperature is decreased rapidly to a already determined annealing temperature of 55 degrees celceius which allows the primer to sit down and become annealed to complementary strand. 1 Question #5: Initialization/Denaturation 2 Question #6: Annealing 3 Question #7: Extension/elongation temperature is increased to 72 degrees c where taq polymerase is most active 4 Question #8: Final elongation the temperature is at 72 degrees celcius for elongation to occur, where taq polymerase adds nucleotides to the growing strand complementary to that of the template 5 Question #13: What structural element from the previous question is no longer rotating upon binding to DNA that leads to a fluorescence signal? 6 Question #15: Speculate on what may have caused more than just the intended fragment to appear in Sample B. phenyl group there is only one band of amplified product which is what was predicted. 1/1