MASS-SPECTROSCOPY →mous Spectroscopy (ms) is He Compounds. used to determine mol mas of → It is most accurate method amongst all other spectroscapie techniques. CHS C-C-CM3 (5M12 (12×5) + (1×12)=60+12=72. CH3 (Neopentane). → Signal will appear in the form of mars to Charge Ratio(m/porn/ → Generally charge will be +1 in most of the caser ; rarely it will bet2. →72/+1 =72) Molecular weight IKINCIPLE: A beam of elections will be bombarded in the analyse Compound & it will leads to removal of le" from analyte. → Due to Removed of the e, molecule will be positively changed. & known al Molecular lon. → Materwar lons will be fragmented. Gas Phase lonization: oldent and Most Popular methard. Atomosphere Atomospherice trasure Chemreal emrahom Presure Photon kuzation Sample is vaporized before lonized. Electron lontration (EI) Direct contration mrough electrom beam. by elchers Chemical lommation ((I)) Carrier fas will be lowned, then secondary lons will be produced & lons will be transfered to the analytes. Desorption Ionization Method:. * Liquid / Selid samples will be directly converted into faseoul land. Field Desorption: Desorption of e from the analyte through anade. Fast Atom Bombardment (FAB): Sample is nuxed with matrix and Neutral atom beam re bombarded. Matix Assisted laser Desorption lonization (MALDI). mixed with Polar matrix & laser light is used for lowration of molerules. Evaporative Ionination method. Solvent molecules are stripped by evaporation. > There echniques are specially used in Chromatography, ELECTROW JONIZATION: → It is also known as electron bombardment louisation, main function of this louration rechnique is to convert the Cejaseous sample into Molecular Ions. -> 9t is a hard lendzation Techniques because it will produre Toen. semization porntial of Organse compounds are approximately 8-15ev. and direct bombardment takes plait, so due to wigh energy Mis technique will produce large no. of fragment lons from mollons.