University:
American Career College
Course:
MA700 | Clinical Procedures and Pharmacology
Academic year:

2021
Views:

291

Pages:

26
Author:

SeaPoke

Laboratory Diagnosis of Amoeba 2 Intestinal protozoa – Amoeba  Entamoeba coli -Trophozoite -Cyst  Entamoeba histolytica -Trophozoite -Cyst 3 Amoeba Nucleus Cytoplasm Endoplasm: the inner layer of the cytoplasm, Endoplasm Cytoplasm which immediately surrounds the nucleus and is moderately dense and granulated. Ectoplasm: Enveloping the endoplasm is the Ectoplasm less granular and more homogenous than endoplasm. 4 Amebae Nucleus Ameba (Entamoeba)  Vesicular nucleus Karyosome (endosome, nucleolus): a single mass or aggregate of granules Peripheral chromatin: chromatin granules may line the nuclear membrane. 5 Development Stages  Trophozoite – vegetative stage  Cyst – infective stage 6 Nine species have been established as parasites of human: Genus: Entamoeba Species: E. histolytica E. dispar E. hartmanni E. coli E. gingivalis E. polecki E. moshkovskii Genus: Endolimax Species: E. nana Genus: Iodamoeba Species: I. bütschlii 7 8 Entamoeba coli  most common endocommensal of humans  has a worldwide distribution  common inhabitant of the lumen of the cecum and colon of human.  Feeds on bacteria and any other cells available to it  does not invade tissues 9 Trophozoites of Entamoeba coli  Size: 15-50 μm  Cytoplasm: There is no clear demarcation between the ectoplasm and endoplasm The endoplasm is highly vacuolated containing bacteria  Nucleus: One spherical nucleus with thick nuclear membrane lined with coarse, irregular chromatin granules or plaques  The karyosome is moderately large and eccentrically located 10 Trophozoites of Entamoeba coli 11 Trophozoite of Entamoeba coli 12 Trophozoites of Entamoeba coli 13 Cyst of Entamoeba coli  Shape: spherical with smooth, definite, thick cystic wall  Size: 10-35 μm  Nucleus: spherical; coarse, irregular peripheral chromatin; a large eccentric karyosome  mature cyst: 8 nuclei Immature cyst: 2 or 4 nuclei  Cytoplasm: coarsely granular In younger cysts, chromatoidal bars with splintered ends or broom-shape ends and glycogen mass 14 Cyst of Entamoeba coli 2 nuclei 15 Cyst of Entamoeba coli 8 nuclei 16 Trophozoites of Entamoeba histolytica  Size:  Minuta: 12-15 μm  Hematophage: 20-60 μm  Cytoplasm: There is clear demarcation between the ectoplasm & endoplasm The endoplasm is dense & fine granular, vacuolated containing bacteria or RBCs  Nucleus: One spherical nucleus with thin nuclear membrane lined with a rim of chromatin that is frequently distributed in small round granules.  The karyosome is small, distinct and central located. 17 Trophozoites of Entamoeba histolytica Trophozoites 18 Trophozoites of Entamoeba histolytica Hematophage Magnata 19 Trophozoites of Entamoeba histolytica Minuta Hematophage 20 Trophozoites of Entamoeba histolytica Hematophage 21 Trophozoites of Entamoeba histolytica Hematophage 22 Trophozoites of Entamoeba histolytica Hematophage 23 Cyst of Entamoeba histolytica  Shape: spherical with smooth, definite, relatively thin cystic wall  Size: 10-20 μm  Nucleus: spherical with a centrally located karyosome & peripheral evenly distributed small chromatin dots.  mature cyst: 4 nuclei Immature cyst: 1 or 2 nuclei  Cytoplasm: fine granular In younger cysts, chromatoidal bars with rounded ends and glycogen mass may be present 24 Cyst of Entamoeba histolytica 25 Cyst of Entamoeba histolytica 26 Cyst of Entamoeba histolytica 27