Pyruvate Oxidation Lecture

Pyruvate Oxidation Enzyme 1 • Step 1: Decarboxylation of pyruvate to an enol (hydroxyethyl-TPPl • Step 2: Acylation of enol to a thioester on lipoic acid. Enzyme 2 • Step 3: Formation of acetyl-CoA: simple thio-ester exchange Enzyme 3 • Step 4: Reoxidation of the lipoamide cofactor; reduction of FAD/Cys/Cys • Step 5: Regeneration of the oxidized FAD/Cys/Cys active site – forming NADH 1 Pyruvate Oxidation E196, E224, E324 E2 Pyruvate Oxidation E196, E224, E324 E2 2 Pyruvate Oxidation Overall Reaction of PDC Pyruvate + Coenzyme-A (CoASH) + NAD+ ⇌ PDC (TPP, lipoic acid, FAD) DG°’ = –8 kcal/mol CO2 + Acetyl-Coenzyme-A (Ac-CoA) + NADH + H+ Fates of Acetyl CoA (HMG-CoA) Amino acids 1. Oxidation 2. Lipid metabolism 3. Amino acid metabolism Pyruvate Oxidation Overall Reaction of PDC Pyruvate + Coenzyme-A (CoASH) + NAD+ ⇌ PDC (TPP, lipoic acid, FAD) DG°’ = –8 kcal/mol CO2 + Acetyl-Coenzyme-A (Ac-CoA) + NADH + H+ Fates of Acetyl CoA (HMG-CoA) Amino acids 1. Oxidation 2. Lipid metabolism 3. Amino acid metabolism 3 The Citric Acid Cycle Glycogen-o-lysis Glyco-lysis Pyruvate Oxidation Krebs' Cycle Phosphorylation Oxidative The Citric Acid Cycle a.k.a. Krebs Cycle, a.k.a. Tricarboxylic Acid Cycle (TCA) 4 Time B.C. (Before the Cycle) Otto Warburg 1883-1970 Manometer Warburg Apparatus Substrates -respiration (e.g., glucose) -Measure rates of O2 consumption UTube instructions CO2 trap CO2 à H2CO3 à 2H+ + CO3-2 à K2CO3 + H2O Tissues Time B.C. (Before the Cycle) O-leic M-alonic S-uccinic G-lutaric A-dipic P-imelic In 1920 BC, what was known about respiration? 1) Glycolysis gives rise to pyruvate 2) Adding pyruvate to respiring tissues in a Warburg apparatus, there are 2.5 O2 consumed: 1/2O 221/2 àà à 3CO + 2H O O ++CC HH OO à àà 3CO + 2H O 22 3) 4) 33 44 33 2 2 2 2 Any intermediate in the process will be oxidized at a rate ≥pyruvate Many intermediates were tried, but few met this criteria, they were: succinate, fumarate, malate, alpha-ketoglutarate, etc. Albert Szent-Györgyi a-ketoglutarate glutarate à succinate à fumarate à malate à oxaloacetate | citrate à aconitate à isocitrate 6) In 1937, with help of German biochemist Franz Koop, Carl Martinus, demonstrated a series of reactions using citrate that produced aketoglutarate. Thus tricarboxylic acid and dicarboxylic acids would be interconverted with loss of CO2, but also support respiration. 1893-1986 5) Others had already worked out several compounds and their interconversion. Specifically, Albert Szent-Györgi had worked out the interconversion of the dicarboxylic acids. Carl Martinus worked out the interconversion of the tricarboxylic acids 1/2 5 Time B.C. (Before the Cycle) O-leic M-alonic S-uccinic G-lutaric glutarate à succinate à fumarate à malate à oxaloacetate A-dipic P-imelic citrate à aconitate à isocitrate “pyruvate” (acetate) Hans Krebs 1900-1981 Krebs confirmed that the pathway was consistent with succinate, fumarate, and malate proved to be useful because all these molecules increased oxygen consumption in the pigeon breast muscle. Dr. Kornberg: Lecture 02.08.17 (19:54-20:39) (1 min) The first clue came from an experiment with fumarate. Krebs did careful measurements using the Warburg manometer. Fumarate gave greater than expected oxygen consumption in the pigeon breast muscle. 3O2 + C4H4O4à4CO2 + 2H2O ∴1 µmole fumarate would consume 3 µmole O2 1)Malonic acid inhibition of the succinate à fumarate step prevented this increase & succinate accumulated 2)How can fumarate give rise to succinate? There must be a cycle 3)Tested by showing that using succinate or fumarate you could detect the formation of citrate. Later in 1937, he proposed that pyruvate would combine with oxaloacetate to make citrate in a cycle he called the Citric Acid Cycle. Later, Fritz Lipmann showed that it was acetyl-CoA and not pyruvate. The Citric Acid Cycle • Step 1: C-C bond formation between acetate (2C) and oxaloacetate (4C) to make citrate (6C) • Step 2: Isomerization via dehydration/rehydration • Steps 3–4: Oxidative decarboxylations to give 2 NADH • Step 5:① Substrate-level phosphorylation to give GTP ② to give FADH2 • Step 6: Dehydrogenation • Step 7: Hydration • Step 8: Dehydrogenation to give NADH ⑧ ③ ⑦ ④ ⑥ ⑤ 6 The Citric Acid Cycle The Citric Acid Cycle: Citrate Synthase • Condensation of acetyl-CoA and oxaloacetate • The only reaction with C-C bond formation • Highly thermodynamically favorable/irreversible (DG°’ = –7.7 kcal/mol) – regulated by substrate availability and product inhibition – OH R2 – • Activity largely depends on [oxaloacetate]. • Rate-limiting step of CAC • Uses acid/base catalysis R2 – Carbonyl of oxaloacetate is a good electrophile. – Methyl of acetyl-CoA is not a good nucleophile… – …unless activated by deprotonation. 7 The Citric Acid Cycle: Citrate Synthase Mechanism • Conformational change occurs upon binding oxaloacetate. • Avoids unnecessary hydrolysis of thioester in acetyl-CoA a) Open conformation: Free enzyme does not have a binding site for acetyl-CoA. Ordered binding. b) Closed conformation: Binding of OAA creates binding for acetylCoA. Reactive carbanion is protected. Acid/Base Catalysis The Citric Acid Cycle: Citrate Synthase Mechanism carbanion 8 The Citric Acid Cycle: Citrate Synthase Mechanism carbanion The Citric Acid Cycle: Citrate Synthase Mechanism Hydrolysis of Thioester; citroyl-CoA 9 The Citric Acid Cycle: Citrate Synthase Mechanism Hydrolysis of Thioester; citroyl-CoA The Citric Acid Cycle: Aconitase Isomerization by Dehydration/Rehydration aconitase •Elimination of H2O from the symmetrical molecule, citrate, gives a cis C=C bond. aconitase – lyase •Rationale: – Citrate, a tertiary alcohol, is a poor substrate for oxidation. – Isocitrate, a secondary alcohol, is a good substrate for oxidation. •Thermodynamically unfavorable/reversible (DG°’ = +3.2 kcal/mol) – product concentration kept low to pull forward; citrate tends to “pool” with higher conc. • Addition of H2O to cis-aconitate is stereospecific. – This was initially very confusing to bio/organic chemists – Only R-isocitrate is produced by aconitase. – A biochemist names A.G. Ogston clarified the situation by realizing that the enzyme spatially templates this symmetrical molecule by binding in only one way (e.g., clockwise or counter clockwise, not both) – Distinguished by three-point attachment to the active site 10