Determination of ESR and PCV

Determination of. Erythrocyte Sedimentation Rate Aim: To assess the rate of Settling of RBCs When anticoagulated blood is allowed •a vertically placed tube led to stand in Principle:- differences in density of RBCs and plasma favours Sedimentation of blood cells at the bottom allowing a clear Supernatant plasma! Requirements:- anticoagulated blood, Westergern's tube and Stand, heintrobe's tube and Stand, timer. Westergren's tabe: - the pipette is a long cylindrical tube of the uniform bore of 2.5 mm diameter and a length of about 300 mm, it is open at both ends and is marked with graduations from 0 to 200mm. Westergren's tube Stand:- it can accommodate 5 pipette there is a rubber cushion at the base on which the lower end of the Westergrens pipette is made to rest. At the upper end there is a screw Cap which rest "upon the pipette this prevents any leakage of blood at the bottom the Stand allows the pipette to remain exactly in a vertical position. Procedure:- Anticoagulant: A solution of 3.8% sodium citrate is used as an anticoagulant, and the ratio between sodium citrate solution is high. and blood should be 1:4, ie. dilute 1 ml of sodium Citrate Solution with 4ml of the given Sample of blood. Sodium citrate is isotonic hlith blood and it is also dilutes. the blood. See that the Westergrens tube is a clean and alry. Dip the slower end of the Westergrens tube Well below the Surface of the sample of the blood and draws the blood Hoully into the tube up to 0. mark. Quickly close the top of the tube with the index finger to the blood from draining out. Take care the coloumn Is free from air bubbles. The level of the blood should be exactly at O and if it exceeds this mark it can be brought to the level by allowing the blood from the lower end with a piece of cotton and place the ☐ tube Vertical in the Stand. Release the finger from the top. Allove the tube to Stand for one hour. Note: the length of clear plasma at the top of the tube in mm. this gives the erythrocyte Sedimentation rate Determination of Packed Cell Volume Aim: To evaluate the per cent value of blood cell Volume and plasma volume of blood. Sample Principle:- By Subjecting a sample of anticoagulant blood taken in a graduated tube (wintrobe's) tube to centrifugation Cell brings heiver than plasma are parked tightly at the lower upper part. part of the tube.. Leaving plasma in the Requirement: Anticoagulated blood, wintrobe's tube, centrifuge, timer, Pasteure pipette. procedure: The packed Cell Volume is determined by using Wintrobe's tube Haematocrit tube is a cylindrical tube closed at one end and marked from the top 0-10 one column and 10-0 on the opposite column oxalated Venous blood is used for the experiment. A mixture of ammonium oxalate and potassium Oxalate in the ratio 3:2 is used as an anticoagulant. The mixture is prepared by dissolved Water. 0.5cc of this Oxalate solution is placed in a small bottle and evaporated to leave the residue of the mixture at the bottom, 2ml of blood is dried from the cubital. vein by a Sterlized Sysing and transferred bottle and mixed well." to the Oxalated The oxalated blood is introduced into winter be's tube Slowly Wing pasteure pipette. The tube is filled up slowly from the bottom, and the pipette is gradually filled up. This ensures that no air bubbles are introduced into the tube Wint robe's tube is filled up to the o mark and placed in a centrifuge and centrifuged at the rate of Soso revolutions per minute for half for half an hour. The tube is taken out and examined. The red cell are packed at the bottom leaving the clear Straw coloured plasma at the top. Between the two layers. a buffy coat is formed. (0.5 to 1 mm thick). This consist of levescytes and platelets.