University:
Capital University
Course:
BIOL 100 | General Biology
Academic year:

2021
Views:

437

Pages:

3
Author:

kundgetanufv

Biology Notes MODULE BIOSL 1: EXPLORE CONTINUITY AND DIVERSITY OF LIFE MICROSCOPY (PC 1.1.1 – 1.1.2)  Microscopes and microbiology  linking advance  The microscope is the microbiologist’s most basic tool.  Microscopes use lenses to magnify object’s images.  There are many types of microscopes (look at the next slide) but the most common include two types: ◦ Light microscopes (5 types) ◦ Electron microscope (2 types)  Light microscope used to examine cells at relatively low magnifications and electron microscope used to lock at cells and cell structure at very high magnification. Principles of Light Microscope  Compound light microscope uses visible light to illuminate cells.  Many different types of light microscopy:  1-Brightfield  2-Darkfield  3-Phase-contrast  4-Differential interference contrast (DIC)  5-Fluorescence Bright-field microscope  Specimens are visualized because of differences in contrast (density) between specimen and surroundings.  Contrast differences arise because cells absorb or scatter light to varying degrees. Two sets of lenses form the image.  Objective lens and ocular lens (compound)  Total magnification = objective magnification  ocular magnification  Maximum magnification is ~2,000

Exploring Continuity and Diversity of Life

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