Activity 5 Simulating Active Transport

PhysioEx Lab Report Exercise 1: Cell Transport Mechanisms and Permeability Activity 5: Simulating Active Transport Name: Date: 15 September 2020 Pre-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. 1 The movement of sodium and potassium maintained by the Na -K pump+ + You correctly answered: requires energy and is against a concentration gradient. 2 The sodium-potassium pump is classified as a(n) You correctly answered: antiporter. 3 The sodium-potassium pump moves _____ sodium ions and _____ potassium ions simultaneously. You correctly answered: 3, 2. 4 Solutes that require active transport for movement might be too large to pass or might be You correctly answered: lipid insoluble. Experiment Results Predict Questions 1 Predict Question 1: What do you think will result from these experimental conditions? + be maximally transported. Your answer: Na will 2 Predict Question 2: Do you think the addition of glucose carriers will affect the transport of sodium or potassium? Your answer: No, it will not affect the transport of either ion. Stop & Think Questions 1 Why did the sodium transport stop before the transport was completed? You correctly answered: The ATP was depleted. 2 Why was the equilibrium for the solutes reached earlier? You correctly answered: There were more pumps for transport. Experiment Data Run Number 1 Solute ATP (mM) Na + Cl + - 1 Start Conc. L (mM) 9.000 0.000 Start Conc. R (mM) Pumps 0.000 500 --- Rate (mM/sec) 0.0188 1 K Cl 1 500 --- 1 Glucose --- 0.000 0.000 --- 0 0.0000 2 Na + Cl - 3 9.000 0.000 500 --- 0.0025 2 K+ Cl- 3 0.000 6.000 500 --- 0.0017 2 Glucose --- 0.000 0.000 --- 0 0.0000 3 Na + Cl - 3 9.000 0.000 500 --- 0.0000 3 K+ Cl- 3 0.000 0.000 500 --- 3 Glucose --- 0.000 0.000 --- 0 0.0000 4 Na + Cl - 3 9.000 0.000 800 --- 0.0042 4 K+ Cl- 3 0.000 6.000 800 --- 4 Glucose --- 0.000 0.000 --- 0 0.0000 5 Na + Cl - 3 9.000 0.000 800 --- 0.0042 5 K+ Cl- 3 0.000 6.000 800 --- 5 Glucose --- 0.000 6.000 Carriers 10.000 --- 400 Post-lab Quiz Results You scored 50% by answering 2 out of 4 questions correctly. 1 What happened when you increased the amount of ATP dispensed with the same concentration of sodium and potassium on either side of the membrane? Your answer: The ions were transported more slowly. Correct answer: More ions were transported. 2 At what concentration of ATP were the sodium and potassium maximally transported? Your answer: 1 mM ATP. Correct answer: 3 mM ATP. + What was the effect of adding more+ Na -K pumps to the simulated cell? 0.0125 0.0000 0.0028 0.0028 0.0028# You correctly answered: Transport of the ions was faster. 4 Describe the effect of adding glucose carriers to the sodium and potassium transport. You correctly answered: There was no change in the transport rate because glucose is transported independently. Review Sheet Results 1 Describe the significance of using 9 mM sodium chloride inside the cell and 6 mM potassium chloride outside the cell, instead of other concentration ratios. Your answer: Knowing that Na/K+ pumps work with a 3:2 ratio, it is ideal to use 9mM:6mM for a concentration ratio. 2 Explain why there was no sodium transport even though ATP was present. How well did the results compare with your prediction? Your answer: Sodium by itself will not make the pump work. Sodium and potassium are needed for Na/K+ pump. 3 Explain why the addition of glucose carriers had no effect on sodium or potassium transport. How well did the results compare with your prediction? Your answer: Since glucose works independently and is NOT necessary, it does not help with sodium transport. 4 Do you think glucose is being actively transported or transported by facilitated diffusion in this experiment? Explain your answer. Your answer: It is transported by active transport in this experiment, due to the amount of ATP dispursed. A carrier is needed to transport glucose across the cell membrane due to it's size, it is too large, and lipid insoluble also.